論文 - 詳細
| RRC ID | 29263 |
|---|---|
| 著者 | Watson D, O'Connell Motherway M, Schoterman MH, van Neerven RJ, Nauta A, van Sinderen D. |
| タイトル | Selective carbohydrate utilization by lactobacilli and bifidobacteria. |
| ジャーナル | J Appl Microbiol |
| Abstract |
AIM:To evaluate the ability of specific carbohydrates, including commercially available products, to support the growth of representatives of two well-known groups of gut commensals, namely lactobacilli and bifidobacteria. METHODS AND RESULTS:Sixty-eight bacterial strains, representing 29 human-derived lactobacilli and 39 bifidobacteria (both human- and animal-derived), were tested for their ability to metabolize 10 different carbohydrates. Analysis of growth and metabolic activity was performed using a combination of diagnostic parameters, such as final OD600 , final pH, fermentation end products and growth rate. CONCLUSIONS:The data assembled in this study provide significant complementary and comparative information on the growth-promoting properties of a range of carbohydrates, while also investigating interspecies differences between lactobacilli and/or bifidobacteria with regard to their carbohydrate utilization abilities. Galacto-oligosaccharides (GOS) and lactulose were shown to support the most favourable growth characteristics, whereas relatively poor growth of lactobacilli and bifidobacteria was observed on inulin, maltodextrin and polydextrose. GOS/inulin (9 : 1) and fructo-oligosaccharides (FOS)/inulin mixtures supported mostly similar growth abilities to those obtained for GOS and FOS, respectively. Microbial consumption of GOS, as determined by high-performance anion-exchange chromatography with pulsed amperometric detection, was evident for both lactobacilli and bifidobacteria. SIGNIFICANCE AND IMPACT OF THE STUDY:These results may allow for the rational prediction of lactobacilli and/or bifidobacteria to be used in conjunction with prebiotics, such as GOS, as synbiotics. |
| 巻・号 | 114(4) |
| ページ | 1132-46 |
| 公開日 | 2013-4-1 |
| DOI | 10.1111/jam.12105 |
| PMID | 23240984 |
| MeSH | Animals Bifidobacterium / growth & development Bifidobacterium / metabolism* Carbohydrate Metabolism* Cluster Analysis Feces / microbiology Fermentation Glucans / metabolism Humans Inulin / metabolism Lactobacillus / growth & development Lactobacillus / metabolism* Oligosaccharides / metabolism Polysaccharides Prebiotics Species Specificity |
| IF | 3.066 |
| 引用数 | 97 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 5820 JCM 7017 JCM 7019 JCM 7027 JCM 7092 JCM 7050 JCM 7052 JCM 7053 JCM 7056 JCM 20097 |