| RRC ID |
29277
|
| 著者 |
Nakamura K, Arakawa K, Kawai Y, Yasuta N, Chujo T, Watanabe M, Iioka H, Tanioka M, Nishimura J, Kitazawa H, Tsurumi K, Saito T.
|
| タイトル |
Food preservative potential of gassericin A-containing concentrate prepared from a cheese whey culture supernatant from Lactobacillus gasseri LA39.
|
| ジャーナル |
Anim Sci J
|
| Abstract |
Gassericin A (GA) is a circular bacteriocin produced by Lactobacillus gasseri LA39. In this study, GA-containing concentrate was prepared using a cross-flow membrane filtration device (30 kDa cut-off) from the culture supernatant of Lb. gasseri LA39 cultivated in a cheese whey-based food-grade medium. The bacteriocin activity titer in the concentrate was 16 times as high as that of the culture supernatant and was completely maintained through each incubation at 4°C for 3 months, 37°C for 2 months, 60°C for 5 h, and 100°C for 30 min. The GA-containing concentrate was used with glycine powder to make custard creams, and then four representative strains of custard cream spoilage bacteria (Bacillus cereus, Lactococcus lactis subsp. lactis, Achromobacter denitrificans and Pseudomonas fluorescens) were individually inoculated at c. 10(3) colony forming units/g in the custard creams. Throughout 30 days of incubation at 30°C, all of the inoculated bacteria were completely inhibited by the combination of 5% (w/w) of the GA-containing concentrate and 0.5% (w/w) glycine. This is the first highly practical application of GA to foods as a biopreservative, and the concentration method and the bacteriocin concentrate would contribute to biopreservation of several foods.
|
| 巻・号 |
84(2)
|
| ページ |
144-9
|
| 公開日 |
2013-2-1
|
| DOI |
10.1111/j.1740-0929.2012.01048.x
|
| PMID |
23384356
|
| MeSH |
Bacteria / drug effects*
Bacteriocins / biosynthesis
Bacteriocins / isolation & purification*
Bacteriocins / pharmacology*
Cheese*
Culture Media / chemistry*
Culture Media / metabolism*
Dose-Response Relationship, Drug
Drug Resistance, Bacterial
Food Microbiology*
Food Preservatives / isolation & purification*
Food Preservatives / pharmacology*
Lactobacillus / metabolism*
Milk Proteins*
Whey Proteins
|
| IF |
1.399
|
| 引用数 |
17
|
|
WOS 分野
|
AGRICULTURE, DAIRY & ANIMAL SCIENCE
|
| リソース情報 |
| 一般微生物 |
JCM 1002
JCM 11064 |
