| RRC ID |
29285
|
| Author |
Zhu W, Xia JL, Yang Y, Nie ZY, Peng AA, Liu HC, Qiu GZ.
|
| Title |
Thermophilic archaeal community succession and function change associated with the leaching rate in bioleaching of chalcopyrite.
|
| Journal |
Bioresour Technol
|
| Abstract |
The community succession and function change of thermophilic archaea Acidianus brierleyi, Metallosphaera sedula, Acidianus manzaensis and Sulfolobus metallicus were studied by denaturing gradient gel electrophoresis (DGGE) analysis of amplifying 16S rRNA genes fragments and real-time qPCR analysis of amplifying sulfur-oxidizing soxB gene associated with chalcopyrite bioleaching rate at different temperatures and initial pH values. The analysis results of the community succession indicated that temperature and initial pH value had a significant effect on the consortium, and S. metallicus was most sensitive to the environmental change, A. brierleyi showed the best adaptability and sulfur oxidation ability and predominated in various leaching systems. Meanwhile, the leaching rate of chalcopyrite closely related to the consortium function embodied by soxB gene, which could prove a desirable way for revealing microbial sulfur oxidation difference and tracking the function change of the consortium, and for optimizing the leaching parameters and improving the recovery of valuable metals.
|
| Volume |
133
|
| Pages |
405-13
|
| Published |
2013-4-1
|
| DOI |
10.1016/j.biortech.2013.01.135
|
| PII |
S0960-8524(13)00167-3
|
| PMID |
23454386
|
| MeSH |
Archaea / genetics*
Archaea / metabolism*
Biodegradation, Environmental
Copper / isolation & purification*
DNA Fingerprinting
Denaturing Gradient Gel Electrophoresis
Gene Expression Regulation, Archaeal
Genes, Archaeal / genetics
Hydrogen-Ion Concentration
Principal Component Analysis
RNA, Ribosomal, 16S / genetics
Temperature*
Water Pollutants, Chemical / isolation & purification*
|
| IF |
7.539
|
| Times Cited |
19
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
AGRICULTURAL ENGINEERING
ENERGY & FUELS
|
| Resource |
| General Microbes |
JCM 8954 |
