RRC ID 29292
著者 Suzuki T, Soga S, Inoue M, Uda K.
タイトル Characterization and origin of bacterial arginine kinases.
ジャーナル Int J Biol Macromol
Abstract Arginine kinase (AK) plays a key role in ATP buffering systems of tissues and nerves that display high and variable rates of ATP turnover and is widely distributed in invertebrate animals. The enzyme is also found in unicellular organisms, protists and bacteria, but its occurrence is intermittent among species. The AK sequence is structurally divided into two domains, N- and C-terminal domains. The purpose of this study is to clarify the origin of bacterial AK. A search of over 1700 bacterial genomic sequences revealed eight species from Deinococcus-Thermus (Oceanithermus profundus) and Proteobacteria (Ahrensia sp., Nitratifractor salsuginis, Desulfobacterium autotrophicum, Desulfotalea psychrophila, Myxococcus xanthus, Moritella sp. and Sulfurovum sp.) possessing a complete AK sequence homologue. In addition, we searched another key protein that is homologous with that of the C-terminal domain of AK (mcsB). The mcsB is more widely distributed in about 150 species across at least nine bacterial genera. In agreement with the report by other authors, a phylogenetic tree of AK homologues shows that the eight species are separated into two clusters: cluster-A with AKs from ciliates Tetrahymena and Sterkiella and a porifera and the larger cluster-B, including most of the invertebrate AKs. We cloned and expressed the AK from Sulfurovum lithotrophicum in cluster-A and determined its enzymatic properties. Bacterial AKs were characterized as having the highest catalytic efficiency among known AKs, although there was a marked difference in kcat values for cluster-A and -B bacterial AKs. These observations suggest that bacterial AKs in cluster-B may be the prototype of invertebrate AKs. On the other hand, it appears that bacterial AKs in cluster-A diverged at an early stage of bacterial evolution after the appearance of AK, or introduced by horizontal gene transfer.
巻・号 57
ページ 273-7
公開日 2013-6-1
DOI 10.1016/j.ijbiomac.2013.02.023
PII S0141-8130(13)00104-9
PMID 23523720
MeSH Arginine Kinase / genetics* Arginine Kinase / metabolism Bacteria / enzymology Bacteria / genetics* Bacterial Proteins / genetics* Bacterial Proteins / metabolism Evolution, Molecular* Gene Transfer, Horizontal / physiology Genome, Bacterial / physiology* Phylogeny*
IF 5.162
引用数 19
WOS 分野 CHEMISTRY, APPLIED POLYMER SCIENCE BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM 12117