RRC ID 29311
Author Liu W, Ren P, He S, Xu L, Yang Y, Gu Z, Zhou Z.
Title Comparison of adhesive gut bacteria composition, immunity, and disease resistance in juvenile hybrid tilapia fed two different Lactobacillus strains.
Journal Fish Shellfish Immunol
Abstract This study compares the effects of two Lactobacillus strains, highly adhesive Lactobacillus brevis JCM 1170 (HALB) and less-adhesive Lactobacillus acidophilus JCM 1132 (LALB), on the survival and growth, adhesive gut bacterial communities, immunity, and protection against pathogenic bacterial infection in juvenile hybrid tilapia. During a 5-week feeding trial the fish were fed a diet containing 0 to 10(9) cells/g feed of the two Lactobacillus strains. Samples of intestine, kidney, and spleen were taken at the start and at 10, 20, and 35 days for analysis of stress tolerance and cytokine gene mRNA levels and to assess the diversity of adhesive gut bacterial communities. A 14-day immersion challenge with Aeromonas hydrophila NJ-1 was also performed following the feeding trial. The results showed no significant differences in survival rate, weight gain, or feed conversion in the different dietary treatments. The adhesive gut bacterial communities were strikingly altered in the fish fed either the HALB or the LALB, but the response was more rapid and substantial with the adhesive strain. The two strains induced similar changes in the patterns (upregulation or downregulation) of intestinal, splenic or kidney cytokine expression, but they differed in the degree of response for these genes. Changes in intestinal HSP70 expression levels coincided with changes in the similarity coefficient of the adhesive gut bacterial communities between the probiotic treatments. The highest dose of the HALB appeared to protect against the toxic effects of immersion in A. hydrophila (P < 0.05). In conclusion, the degree to which Lactobacillus strains adhere to the gut may be a favorable criterion in selecting probiotic strain for aquaculture.
Volume 35(1)
Pages 54-62
Published 2013-7-1
DOI 10.1016/j.fsi.2013.04.010
PII S1050-4648(13)00499-3
PMID 23608032
MeSH Aeromonas hydrophila / physiology Animal Nutritional Physiological Phenomena Animals Aquaculture Cytokines / genetics Cytokines / metabolism Digestion Disease Resistance / drug effects Fish Diseases / immunology* Fish Diseases / mortality Fish Diseases / prevention & control Gene Expression Regulation Gram-Negative Bacterial Infections / immunology Gram-Negative Bacterial Infections / mortality Gram-Negative Bacterial Infections / prevention & control Gram-Negative Bacterial Infections / veterinary* Hybridization, Genetic Immunity, Innate / drug effects Intestines / microbiology Lactobacillus acidophilus / metabolism* Lactobacillus brevis / metabolism* Longevity Organ Specificity Probiotics / administration & dosage* Tilapia / growth & development Tilapia / immunology* Tilapia / microbiology Tilapia / physiology Weight Gain
IF 3.298
Times Cited 72
General Microbes JCM 1132 JCM 1170