| RRC ID |
29352
|
| 著者 |
Qiu XX, Mou YZ, Zhao ML, Zhang WJ, Han D, Ren M, Cui HL.
|
| タイトル |
Halobellus inordinatus sp. nov., from a marine solar saltern and an inland salt lake of China.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Two halophilic archaeal strains, YC20(T) and XD15, were isolated from a marine solar saltern and an inland salt lake in China. Both had pleomorphic cells that lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. They were neutrophilic, requiring at least 100 g NaCl l(-1) and 0.5-95 g MgCl2 l(-1) for growth at the optimum growth temperature of 37 °C. The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS) and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1), respectively. Trace amounts of two unidentified glycolipids were also detected. The 16S rRNA gene sequences of the two strains were 99.5 % identical and showed 94.0-95.9 % similarity to the most closely related members of the genus Halobellus of the family Halobacteriaceae. The rpoB' gene sequence similarity between strains YC20(T) and XD15 was 98.2 % and these sequences showed 89.6-92.8 % similarity to those of the most closely related members of the genus Halobellus. The DNA G+C contents of strains YC20(T) and XD15 were 65.8 mol% and 65.4 mol%, respectively. The DNA-DNA hybridization value between strain YC20(T) and strain XD15 was 92 %, and the two strains showed low DNA-DNA relatedness to members of the genus Halobellus. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains YC20(T) and XD15 represent a novel species of the genus Halobellus, for which the name Halobellus inordinatus sp. nov. is proposed. The type strain is YC20(T) ( = CGMCC 1.12120(T) = JCM 18361(T)) and the other strain is XD15 ( = CGMCC 1.12236 = JCM 18648).
|
| 巻・号 |
63(Pt 11)
|
| ページ |
3975-3980
|
| 公開日 |
2013-11-1
|
| DOI |
10.1099/ijs.0.053066-0
|
| PMID |
23728369
|
| MeSH |
Base Composition
China
DNA, Archaeal / genetics
Genes, Archaeal
Glycolipids / chemistry
Halobacteriaceae / classification*
Halobacteriaceae / genetics
Halobacteriaceae / isolation & purification
Lakes / microbiology*
Magnesium Chloride
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Salts
Sodium Chloride
Temperature
Water Microbiology*
|
| IF |
2.415
|
| 引用数 |
13
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 9738
JCM 18361
JCM 18648 |
