論文 - 詳細
| RRC ID | 29385 |
|---|---|
| 著者 | Shimizu S, Ueno A, Tamamura S, Naganuma T, Kaneko K. |
| タイトル | Methanoculleus horonobensis sp. nov., a methanogenic archaeon isolated from a deep diatomaceous shale formation. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A methanogenic organism from the domain Archaea, designated strain T10(T), was isolated from groundwater sampled from a deep diatomaceous shale formation located in Horonobe, Hokkaido, Japan. The strain utilized H2/CO2 and formate as substrates for methanogenesis. Cells were strictly anaerobic, Gram-negative-staining, flagellated, irregular coccoids, 0.7-1.6 µm in diameter, and occurred singly. The strain grew at 25-45 °C (optimum 37-42 °C), at pH 5.8-8.2 (optimum pH 6.7-6.8) and in the presence of 0-1.3 M NaCl (optimum 0.1-0.2 M NaCl). The G+C content of the genomic DNA was 62.9 mol%. 16S rRNA gene sequencing revealed that, although the strain is a member of the genus Methanoculleus, it clearly differed from all described species of this genus (95.5-98.3 % sequence similarity). Values for DNA-DNA hybridization with type strains of closely related Methanoculleus species were less than 50 %. Phenotypic and phylogenetic features of strain T10(T) clearly indicate that it represents a novel species of the genus Methanoculleus, for which the name Methanoculleus horonobensis sp. nov. is proposed. The type strain is T10(T) ( = DSM 21626(T) = JCM 15517(T)). |
| 巻・号 | 63(Pt 11) |
| ページ | 4320-4323 |
| 公開日 | 2013-11-1 |
| DOI | 10.1099/ijs.0.053520-0 |
| PMID | 23832970 |
| MeSH | Base Composition DNA, Archaeal / genetics Groundwater / microbiology* Japan Methanomicrobiaceae / classification* Methanomicrobiaceae / genetics Methanomicrobiaceae / isolation & purification Molecular Sequence Data Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA |
| IF | 2.415 |
| 引用数 | 13 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 15517 |