| RRC ID |
29676
|
| 著者 |
Jiménez-Hidalgo M, Kurz CL, Pedrajas JR, Naranjo-Galindo FJ, González-Barrios M, Cabello J, Sáez AG, Lozano E, Button EL, Veal EA, Fierro-González JC, Swoboda P, Miranda-Vizuete A.
|
| タイトル |
Functional characterization of thioredoxin 3 (TRX-3), a Caenorhabditis elegans intestine-specific thioredoxin.
|
| ジャーナル |
Free Radic Biol Med
|
| Abstract |
Thioredoxins are a class of evolutionarily conserved proteins that have been demonstrated to play a key role in many cellular processes involving redox reactions. We report here the genetic and biochemical characterization of Caenorhabditis elegans TRX-3, the first metazoan thioredoxin with an intestine-specific expression pattern. By using green fluorescent protein reporters we have found that TRX-3 is expressed in both the cytoplasm and the nucleus of intestinal cells, with a prominent localization at the apical membrane. Although intestinal function, reproductive capacity, longevity, and resistance of trx-3 loss-of-function mutants to many stresses are indistinguishable from those of wild-type animals, we have observed a slight reduction in size and a minor reduction in the defecation cycle timing of trx-3 mutants. Interestingly, trx-3 is induced upon infection by Photorhabdus luminescens and Candida albicans, and TRX-3 overexpression provides a modest protection against these pathogens. Together, our data indicate that TRX-3 function in the intestine is dispensable for C. elegans development but may be important to fight specific bacterial and fungal infections.
|
| 巻・号 |
68(100)
|
| ページ |
205-19
|
| 公開日 |
2014-3-1
|
| DOI |
10.1016/j.freeradbiomed.2013.11.023
|
| PII |
S0891-5849(13)01528-1
|
| PMID |
24316195
|
| PMC |
PMC4018987
|
| MeSH |
Amino Acid Sequence
Animals
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / genetics*
Intestinal Mucosa / metabolism*
Longevity / genetics
Organ Specificity
Oxidation-Reduction
Thioredoxins / biosynthesis*
Thioredoxins / genetics
|
| IF |
6.17
|
| 引用数 |
9
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| 線虫 |
tm2820 |
