論文 - 詳細
| RRC ID | 29676 |
|---|---|
| 著者 | Jiménez-Hidalgo M, Kurz CL, Pedrajas JR, Naranjo-Galindo FJ, González-Barrios M, Cabello J, Sáez AG, Lozano E, Button EL, Veal EA, Fierro-González JC, Swoboda P, Miranda-Vizuete A. |
| タイトル | Functional characterization of thioredoxin 3 (TRX-3), a Caenorhabditis elegans intestine-specific thioredoxin. |
| ジャーナル | Free Radic Biol Med |
| Abstract |
Thioredoxins are a class of evolutionarily conserved proteins that have been demonstrated to play a key role in many cellular processes involving redox reactions. We report here the genetic and biochemical characterization of Caenorhabditis elegans TRX-3, the first metazoan thioredoxin with an intestine-specific expression pattern. By using green fluorescent protein reporters we have found that TRX-3 is expressed in both the cytoplasm and the nucleus of intestinal cells, with a prominent localization at the apical membrane. Although intestinal function, reproductive capacity, longevity, and resistance of trx-3 loss-of-function mutants to many stresses are indistinguishable from those of wild-type animals, we have observed a slight reduction in size and a minor reduction in the defecation cycle timing of trx-3 mutants. Interestingly, trx-3 is induced upon infection by Photorhabdus luminescens and Candida albicans, and TRX-3 overexpression provides a modest protection against these pathogens. Together, our data indicate that TRX-3 function in the intestine is dispensable for C. elegans development but may be important to fight specific bacterial and fungal infections. |
| 巻・号 | 68(100) |
| ページ | 205-19 |
| 公開日 | 2014-3-1 |
| DOI | 10.1016/j.freeradbiomed.2013.11.023 |
| PII | S0891-5849(13)01528-1 |
| PMID | 24316195 |
| PMC | PMC4018987 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Intestinal Mucosa / metabolism* Longevity / genetics Organ Specificity Oxidation-Reduction Thioredoxins / biosynthesis* Thioredoxins / genetics |
| IF | 6.17 |
| 引用数 | 9 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2820 |