Reference - Detail
| RRC ID | 29697 |
|---|---|
| Author | Xu J, Yoshimura K, Mon H, Li Z, Zhu L, Iiyama K, Kusakabe T, Lee JM. |
| Title | Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells. |
| Journal | Mol Biotechnol |
| Abstract |
The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity. |
| Volume | 56(3) |
| Pages | 193-8 |
| Published | 2014-3-1 |
| DOI | 10.1007/s12033-013-9694-0 |
| PMID | 23979877 |
| MeSH | Animals Biological Transport Bombyx / cytology* Bombyx / metabolism Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / physiology* Cells, Cultured Gene Transfer Techniques* Membrane Proteins / physiology* Plasmids / genetics Plasmids / metabolism RNA, Double-Stranded / genetics* RNA, Double-Stranded / metabolism Transfection |
| IF | 2.022 |
| Times Cited | 7 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Silkworms | |