| RRC ID |
29697
|
| Author |
Xu J, Yoshimura K, Mon H, Li Z, Zhu L, Iiyama K, Kusakabe T, Lee JM.
|
| Title |
Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells.
|
| Journal |
Mol Biotechnol
|
| Abstract |
The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.
|
| Volume |
56(3)
|
| Pages |
193-8
|
| Published |
2014-3-1
|
| DOI |
10.1007/s12033-013-9694-0
|
| PMID |
23979877
|
| MeSH |
Animals
Biological Transport
Bombyx / cytology*
Bombyx / metabolism
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / physiology*
Cells, Cultured
Gene Transfer Techniques*
Membrane Proteins / physiology*
Plasmids / genetics
Plasmids / metabolism
RNA, Double-Stranded / genetics*
RNA, Double-Stranded / metabolism
Transfection
|
| IF |
2.022
|
| Times Cited |
7
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Silkworms |
|
