RRC ID 29747
著者 Fletcher G, Irwin CA, Henson JM, Fillingim C, Malone MM, Walker JR.
タイトル Identification of the Escherichia coli cell division gene sep and organization of the cell division-cell envelope genes in the sep-mur-ftsA-envA cluster as determined with specialized transducing lambda bacteriophages.
ジャーナル J Bacteriol
Abstract From a lysogen with lambda integrated in the leu operon, specialized transducing phages that carry the cell division, murein biosynthesis, and envelope permeability genes located about 0.5 min to the right of leu were isolated. These phages were used to identify the previously undiscovered cell division gene sep. A genetic map proves that sep is located in the sequence leuA sep murE murF murC ddl ftsA envA. A physical map of this region was prepared by heteroduplex analysis of the phage DNAs. Overlapping segments of host DNA extended rightward for as much as 26.4 kilobase pairs from the prophage insertion point (thought to be in leuA) to include all the genes through envA.
巻・号 133(1)
ページ 91-100
公開日 1978-1-1
DOI 10.1128/jb.133.1.91-100.1978
PMID 338600
PMC PMC221981
MeSH Cell Division Chromosome Mapping Coliphages Escherichia coli / genetics* Genes* Genetic Linkage Leucine / metabolism Microscopy, Electron Operon Transduction, Genetic
IF 3.006
リソース情報
原核生物(大腸菌) JE6658 JE6656