RRC ID 29749
Author Ohtsubo E, Hsu MT.
Title Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB.
Journal J. Bacteriol.
Abstract The genetic and physical structures of commonly used F-prime factors carrying the galactose region of the Escherichia coli chromosome were analyzed. Deletions in the chromosomal DNA sequences in the F-prime factors were found to be frequent events. A genetic method was developed to reconstruct the original F-prime factors from deletion variants. Heteroduplex analysis of the reconstructed F-prime factors confirmed the derivation of the F-prime factors F100 and F152, from the same Hfr, and finally determined the normal E. coli chromosomal sequence in the region between fep and uvrB, containing about 5 min in genetic units and about 246.5 in kilobase units (kb). This sequence could be connected with the DNA sequences of the lac-purE region, which had been physically determined previously. Together they constituted a total of 528.6 kb. From these combined sequences, the distance from lacPO to galK was calculated to be 412.9 kb, which corresponds to 8.8 min in genetic units.
Volume 134(3)
Pages 778-94
Published 1978-6
PMID 350847
PMC PMC222324
MeSH Chromosome Mapping Chromosomes, Bacterial* DNA, Bacterial / analysis DNA, Bacterial / genetics* Escherichia coli / genetics* Escherichia coli / ultrastructure F Factor* Microscopy, Electron Molecular Weight
IF 3.234
Prokaryotes E. coli ME8619