RRC ID 29762
Author Kogoma T, Torrey TA, Connaughton MJ.
Title Induction of UV-resistant DNA replication in Escherichia coli: induced stable DNA replication as an SOS function.
Journal Mol Gen Genet
Abstract The striking similarity between the treatments that induce SOS functions and those that result in stable DNA replication (continuous DNA replication in the absence of protein synthesis) prompted us to examine the possibility of stable DNA replication being a recA+ lexA+-dependent SOS function. In addition to the treatments previously reported, ultraviolet (UV) irradiation or treatment with mitomycin C was also found to induce stable DNA replication. The thermal treatment of tif-1 strains did not result in detectable levels of stable DNA replication, but nalidixic acid readily induced the activity in these strains. The induction of stable DNA replication with malidixic acid was severely suppressed in tif-1 lexA mutant strains. The inhibitory activity of lexA3 was negated by the presence of the spr-51 mutation, an intragenic suppressor of lexA3. Induced stable DNA replication was found to be considerably more resistant to UV irradiation than normal replication both in a uvrA6 strain and a uvr+ strain. The UV-resistant replication occurred mostly in the semiconservative manner. The possible roles of stable DNA replication in repair of damaged DNA are discussed.
Volume 176(1)
Pages 1-9
Published 1979-10-2
DOI 10.1007/BF00334288
PMID 396443
MeSH Bacterial Proteins / physiology DNA Repair DNA Replication / radiation effects* Escherichia coli / genetics Escherichia coli / radiation effects* Mutation Thymine / metabolism Ultraviolet Rays
Prokaryotes E. coli ME9100 ME9104 ME9101 ME9102 ME9105