RRC ID 29772
Author Borck K, Beggs JD, Brammar WJ, Hopkins AS, Murray NE.
Title The construction in vitro of transducing derivatives of phage lambda.
Journal Mol Gen Genet
Abstract Methods are described for the construction of plaque-forming, transducing derivatives of phage lambda, using appropirate receptor genomes and fragments of DNA generated by the restriction enzymes endo R.EcoRI and endo R.HindIII. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, sup F, hsd, tna and lig genes have been isolated.
Volume 146(2)
Pages 199-207
Published 1976-7-23
DOI 10.1007/BF00268089
PMID 785220
MeSH Chromosomes, Bacterial Coliphages / metabolism* DNA, Bacterial DNA, Viral Endonucleases / metabolism* Escherichia coli / enzymology* Genetic Techniques Transduction, Genetic*
Resource
Prokaryotes E. coli ME6179