Reference - Detail
| RRC ID | 29780 |
|---|---|
| Author | Timmis K, Cabello F, Cohen SN. |
| Title | Cloning, isolation, and characterization of replication regions of complex plasmid genomes. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
EcoRI endonuclease-generated DNA fragments carrying replication regions of the F'lac and R6-5 plasmids have been cloned and isolated, using as a selection vehicle a nonreplicating ampicillin-resistance DNA fragment derived from a Staphylococcus aureus plasmid. Heteroduplex analysis of the constructed plasmid chimeras and the parent replicons has localized the cloned R6-5 replication region to a DNA segment between kilobase pair coordinates 1.0 and 88.0 on the R6-5 map. Physical proximity between the plasmid replication functions and the locus governing plasmid incompatibility has been shown for both parent replicons. The cloning method reported appears to be generally applicable for the identification and isolation of replication regions of a variety of complex genomes. |
| Volume | 72(6) |
| Pages | 2242-6 |
| Published | 1975-6-1 |
| DOI | 10.1073/pnas.72.6.2242 |
| PMID | 1094460 |
| PMC | PMC432733 |
| MeSH | Centrifugation, Density Gradient DNA Replication* DNA Restriction Enzymes DNA, Bacterial / isolation & purification* Electrophoresis, Agar Gel Escherichia coli / analysis* Escherichia coli / ultrastructure Extrachromosomal Inheritance* Genetics, Microbial Hybridization, Genetic Microscopy, Electron Molecular Weight Phenotype Plasmids* Selection, Genetic Staphylococcus Transformation, Genetic |
| IF | 9.412 |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Prokaryotes E. coli | ME6171 ME6172 ME6163 |