| RRC ID |
29796
|
| 著者 |
Niki H, Jaffé A, Imamura R, Ogura T, Hiraga S.
|
| タイトル |
The new gene mukB codes for a 177 kd protein with coiled-coil domains involved in chromosome partitioning of E. coli.
|
| ジャーナル |
EMBO J
|
| Abstract |
An Escherichia coli temperature sensitive mutant which produces spontaneously normal size anucleate cells at low temperature was isolated. The mutant is defective in a previously undescribed gene, named mukB, located at 21 min on the chromosome. The mukB gene codes for a large protein (approximately 180 kd). A 1534 amino acid protein (176,826 daltons) was deduced from the nucleotide sequence of the mukB gene. Computer analysis revealed that the predicted MukB protein has distinct domains: an amino-terminal globular domain containing a nucleotide binding sequence, a central region containing two alpha-helical coiled-coil domains and one globular domain, and a carboxyl-terminal globular domain which is rich in Cys, Arg and Lys. A 180 kd protein detected in wild-type cell extracts by electrophoresis is absent in mukB null mutants. Although the null mutants are not lethal at low temperature, the absence of MukB leads to aberrant chromosome partitioning. At high temperature the mukB null mutants cannot form colonies and many nucleoids are distributed irregularly along elongated cells. We conclude that the MukB protein is required for chromosome partitioning in E. coli.
|
| 巻・号 |
10(1)
|
| ページ |
183-93
|
| 公開日 |
1991-1-1
|
| DOI |
10.1002/j.1460-2075.1991.tb07935.x
|
| PMID |
1989883
|
| PMC |
PMC452628
|
| MeSH |
Amino Acid Sequence
Bacterial Proteins / genetics*
Base Sequence
Chromosomal Proteins, Non-Histone*
Chromosomes, Bacterial / physiology*
Cloning, Molecular
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification
Escherichia coli / cytology
Escherichia coli / genetics*
Escherichia coli Proteins*
Genes, Bacterial*
Genotype
Molecular Sequence Data
Molecular Weight
Mutation
Protein Conformation
Restriction Mapping
Sequence Homology, Nucleic Acid
|
| IF |
9.889
|
| 引用数 |
321
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME7792 |
