Reference - Detail
| RRC ID | 29797 |
|---|---|
| Author | Miyamoto K, Nakahigashi K, Nishimura K, Inokuchi H. |
| Title | Isolation and characterization of visible light-sensitive mutants of Escherichia coli K12. |
| Journal | J Mol Biol |
| Abstract |
Six mutants of Escherichia coli K12 that are sensitive to visible light have been isolated. Five of them, including an amber mutant, are defective in a gene that maps near 11 minutes on the linkage map of the chromosome, and this gene has been designated visA. The sixth mutant, which was isolated from bacteria that carried the visA+/visA+ diploid allele, is defective in a gene that maps near 63 minutes on the linkage map, which has been designated visB. These mutant strains of bacteria are killed by illumination with visible light. The effective wavelength of the light is around 460 nm. The nucleotide sequence of the visA gene was determined. As a result of a search for homologous products, we found that visA may be identical to hemH, the structural gene for ferrochelatase which catalyzes a final step in the biosynthesis of heme. A possible mechanism for the killing of the visA mutant bacteria is discussed. |
| Volume | 219(3) |
| Pages | 393-8 |
| Published | 1991-6-5 |
| DOI | 10.1016/0022-2836(91)90180-e |
| PII | 0022-2836(91)90180-E |
| PMID | 2051480 |
| MeSH | Amino Acid Sequence Base Sequence Chromosomes, Bacterial* Deoxyribonuclease EcoRI Escherichia coli / genetics* Escherichia coli / isolation & purification Escherichia coli / radiation effects Genes, Bacterial* Light Molecular Sequence Data Mutation* Open Reading Frames Restriction Mapping Saccharomyces cerevisiae / genetics Sequence Homology, Nucleic Acid Software |
| IF | 4.76 |
| Times Cited | 90 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | IH68 |