Reference - Detail
| RRC ID | 297 |
|---|---|
| Author | Horiuchi T, Giniger E, Aigaki T. |
| Title | Alternative trans-splicing of constant and variable exons of a Drosophila axon guidance gene, lola. |
| Journal | Genes Dev |
| Abstract |
longitudinals lacking (lola) is a complex Drosophila gene encoding at least 20 protein isoforms,each bearing the same N-terminal constant region linked to a different C-terminal variable region. Different isoforms specify different aspects of axon growth and guidance. We show here that lola mRNAs are generated by alternative trans-splicing of exons sequentially encoded by the same DNA strand. Chromosomal pairing facilitates interallelic trans-splicing,allowing complementation between mutations in the constant and those in the variable exons. We demonstrate that at least one variable exon is transcribed from its own promoter,and trans-spliced to the constant exons transcribed separately. |
| Volume | 17(20) |
| Pages | 2496-501 |
| Published | 2003-10-15 |
| DOI | 10.1101/gad.1137303 |
| PII | 1137303 |
| PMID | 14522953 |
| PMC | PMC218142 |
| MeSH | Alternative Splicing* Animals Drosophila / genetics* Drosophila Proteins / genetics* Polymorphism, Single Nucleotide Promoter Regions, Genetic Protein Isoforms Sequence Analysis, DNA Transcription Factors / genetics* |
| IF | 9.527 |
| Times Cited | 59 |
| WOS Category | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| Resource | |
| Drosophila | |