| RRC ID |
29806
|
| 著者 |
Kusukawa N, Yura T, Ueguchi C, Akiyama Y, Ito K.
|
| タイトル |
Effects of mutations in heat-shock genes groES and groEL on protein export in Escherichia coli.
|
| ジャーナル |
EMBO J
|
| Abstract |
Escherichia coli heat-shock proteins GroES and GroEL are essential cytoplasmic proteins, which have been termed 'chaperonins' because of their ability to assist protein assembly of bacteriophage capsids and multimeric enzymes of foreign origin. In this report we show that temperature-sensitive mutations in groES and groEL genes cause defective export of the plasmid-encoded beta-lactamase (Bla) in vivo. Since efficient translocation of proteins across biological membranes is thought to be supported by cytoplasmic factors that protect presecretory molecules from being misfolded, these results suggest that both GroES and GroEL proteins possess a chaperone function by which they facilitate export of Bla. The translocation of other secretory proteins, however, appears to depend minimally on GroE, suggesting that GroE interacts only with a specific class of secreted proteins.
|
| 巻・号 |
8(11)
|
| ページ |
3517-21
|
| 公開日 |
1989-11-1
|
| DOI |
10.1002/j.1460-2075.1989.tb08517.x
|
| PMID |
2573517
|
| PMC |
PMC401509
|
| MeSH |
Antigens, Bacterial / genetics*
Antigens, Bacterial / metabolism
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism*
Biological Transport
Cell Fractionation
Chaperonin 10
Chaperonin 60
DNA Mutational Analysis
Escherichia coli / genetics*
Escherichia coli / metabolism
Genes, Bacterial*
Heat-Shock Proteins / genetics*
Heat-Shock Proteins / metabolism
Mutation
Protein Precursors / metabolism
Temperature
beta-Lactamases / metabolism
|
| IF |
9.889
|
| 引用数 |
224
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME7963 |
