Reference - Detail
| RRC ID | 29807 |
|---|---|
| Author | Lech KF, Lee CH, Isberg RR, Syvanen M. |
| Title | New gene in Escherichia coli K-12 (drpA): does its product play a role in RNA synthesis? |
| Journal | J Bacteriol |
| Abstract |
The mutation drpA1 defines a new gene in Escherichia coli K-12 that maps at about 5.2 min. This mutation was obtained after enriching a population of cells for temperature sensitive dna mutations with the [3H]thymidine "suicide" technique followed by screening for mutants defective in transposon Tn5 precise excision. When growing cells carrying the drpA1 allele were shifted to the nonpermissive temperature, we showed that DNA, RNA, and protein syntheses shut off quickly, with the cessation of RNA synthesis occurring first. A recombinant plasmid between pBR322 and an HindIII fragment from wild-type E. coli restores the growth defect in drpA1 mutants. Using transposon Tn5 mutagenesis of this plasmid, we have been able to correlate the presence of a 68-kilodalton protein, as observed with the maxicell technique, with the ability of this plasmid to restore growth to drpA1 mutants. |
| Volume | 162(1) |
| Pages | 117-23 |
| Published | 1985-4-1 |
| DOI | 10.1128/jb.162.1.117-123.1985 |
| PMID | 2579939 |
| PMC | PMC218963 |
| MeSH | Bacterial Proteins / biosynthesis Chromosome Mapping DNA, Bacterial / biosynthesis Escherichia coli / genetics* Genes, Bacterial* Mutation Plasmids RNA, Bacterial / biosynthesis* |
| IF | 3.006 |
| Times Cited | 5 |
| WOS Category | MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Prokaryotes E. coli | ME8379 ME8380 ME8381 |