RRC ID 29811
著者 Kogoma T, Kline BC.
タイトル Integrative suppression of dnaA(Ts) mutations mediated by plasmid F in Escherichia coli is a DnaA-dependent process.
ジャーナル Mol Gen Genet
Abstract The thermosensitivity of dnaA(Ts) mutations can be suppressed by integration of plasmid F (integrative suppression). In the light of the recent finding that F requires DnaA protein for both establishment and maintenance, integrative suppression of 11 dnaA(Ts) mutations by a mini-F, pML31, integrated near oriC was examined. The plating efficiency of integratively suppressed strains was dnaA(Ts) allele-dependent and medium-dependent. The initiation capability of suppressed dnaA(Ts) strains lacking the oriC site and their F- counterparts was determined at various temperatures between 30 degrees C and 42 degrees C. The degree of integrative suppression measured by the initiation capability varied in a dnaA(Ts) allele-dependent manner. F-directed DNA replication was most affected by the dnaA(Ts) mutations mapping in the middle of the gene whereas oriC-dependent replication was most thermosensitive in strains carrying mutations mapping in the carboxy-terminal half of the gene. The results indicated that the integrative suppression by F plasmid is a DnaA-dependent process and suggested that the requirements for DnaA protein in the oriC-dependent replication and F replication processes are qualitatively different.
巻・号 210(2)
ページ 262-9
公開日 1987-12-1
DOI 10.1007/BF00325692
PMID 2830456
MeSH Bacterial Proteins / genetics* DNA Replication* DNA, Bacterial / biosynthesis Escherichia coli / genetics* Escherichia coli / metabolism Genes, Bacterial Mutation Plasmids* Suppression, Genetic* Temperature
引用数 14
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME9286 ME9287 ME9288 ME9294 ME9289 ME9291 ME9292 ME9293 ME9283 ME9284 ME9285 ME9313 ME9295 ME9296 ME9321 ME9322 ME9280 ME9314 ME9315 ME9318 ME9319 ME9320 ME9316 ME9309 ME9312 ME9317 ME9299 ME9300 ME9301 ME9302 ME9303 ME9304 ME9305 ME9306 ME9297 ME9298