論文 - 詳細
| RRC ID | 29816 |
|---|---|
| 著者 | Reitzer LJ, Magasanik B. |
| タイトル | Expression of glnA in Escherichia coli is regulated at tandem promoters. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
We have determined that the glnA gene of the complex glnALG operon of Escherichia coli is transcribed from tandem promoters. Expression from the upstream promoter, glnAp1, requires the catabolite activating protein, is repressed by nitrogen regulator I (NRI), the product of glnG, and produces a transcript with an untranslated leader of 187 nucleotides. Expression from the downstream promoter, glnAp2, requires NRI as well as the glnF product; full expression also requires growth in a nitrogen-limited environment. The downstream transcript has an untranslated leader of 73 nucleotides. We also provide evidence that the function of the glnL product is to mediate the interconversion of NRI between a form capable of activating glnAp2 and an inactive form in response to changes in the intracellular concentration of ammonia. The function of the two minor promoters of the glnALG operon, glnAp1 and glnLp, is to maintain the products of glnA, glutamine synthetase, an essential enzyme, and of glnG, NRI, an activator of nitrogen-controlled genes, during carbon-limited growth. |
| 巻・号 | 82(7) |
| ページ | 1979-83 |
| 公開日 | 1985-4-1 |
| DOI | 10.1073/pnas.82.7.1979 |
| PMID | 2858855 |
| PMC | PMC397465 |
| MeSH | Base Sequence Escherichia coli / enzymology* Escherichia coli / genetics Glutamate-Ammonia Ligase / genetics* Mutation Operon* Transcription, Genetic |
| IF | 9.412 |
| 引用数 | 192 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME8458 |