RRC ID |
29827
|
Author |
Marsh L, Walker GC.
|
Title |
Cold sensitivity induced by overproduction of UmuDC in Escherichia coli.
|
Journal |
J Bacteriol
|
Abstract |
The UmuD and UmuC proteins of Escherichia coli are essential for mutagenesis by UV and most chemicals. Their mode of action is presently unknown. Strains which lack the LexA repressor [lexA(Def)] and contain a pBR322-derived plasmid carrying the umuDC operon overexpress UmuD and UmuC and become cold sensitive (growth at 42 degrees C but not at 30 degrees C). Deletion mapping showed that the umuDC locus on the plasmid is responsible for conferring cold sensitivity. The conditional lethality appeared due to a rapid and reversible inhibition of DNA synthesis at the nonpermissive temperature. Cold sensitivity was enhanced by the increase of NaCl in the medium to 1% and eliminated by 4% ethanol in the medium. Cold sensitivity was partially suppressed by the lon-100 mutation and completely suppressed by the htpR165 mutation.
|
Volume |
162(1)
|
Pages |
155-61
|
Published |
1985-4-1
|
DOI |
10.1128/jb.162.1.155-161.1985
|
PMID |
2984171
|
PMC |
PMC218968
|
MeSH |
Bacterial Proteins / biosynthesis
Bacterial Proteins / genetics*
Cold Temperature*
DNA, Bacterial / biosynthesis
Escherichia coli / genetics*
Escherichia coli / growth & development
Mutation*
|
IF |
3.006
|
Times Cited |
106
|
WOS Category
|
MICROBIOLOGY
|
Resource |
Prokaryotes E. coli |
ME8384
ME8383 |