Reference - Detail
| RRC ID | 29828 |
|---|---|
| Author | Yanisch-Perron C, Vieira J, Messing J. |
| Title | Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. |
| Journal | Gene |
| Abstract |
Three kinds of improvements have been introduced into the M13-based cloning systems. (1) New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors. Mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences. A new suppressorless strain facilitates the cloning of selected recombinants. (2) The complete nucleotide sequences of the M13mp and pUC vectors have been compiled from a number of sources, including the sequencing of selected segments. The M13mp18 sequence is revised to include the G-to-T substitution in its gene II at position 6 125 bp (in M13) or 6967 bp in M13mp18. (3) M13 clones suitable for sequencing have been obtained by a new method of generating unidirectional progressive deletions from the polycloning site using exonucleases HI and VII. |
| Volume | 33(1) |
| Pages | 103-19 |
| Published | 1985-1-1 |
| DOI | 10.1016/0378-1119(85)90120-9 |
| PII | 0378-1119(85)90120-9 |
| PMID | 2985470 |
| MeSH | Base Sequence Cloning, Molecular* Coliphages / genetics* Conjugation, Genetic DNA Restriction Enzymes / metabolism DNA, Single-Stranded / genetics Escherichia coli / metabolism Genetic Vectors* Methylation Mutation Plasmids Recombination, Genetic |
| IF | 2.984 |
| Times Cited | 14785 |
| WOS Category | GENETICS & HEREDITY |
| Resource | |
| Prokaryotes E. coli | ME8649 ME9041 ME8572 pUC18 pUC19 |