RRC ID 29841
Author Stahl FW, Kobayashi I, Thaler D, Stahl MM.
Title Direction of travel of RecBC recombinase through bacteriophage lambda DNA.
Journal Genetics
Abstract We examined linkage relationships for RecBC-mediated recombination in lytic cycle crosses of lambda phages bearing two cohesive end sites (cos) oriented in the same direction. The relationships obtained imply that a given recombinant tends to be packaged from the cos site that is the nearer one to the right of the exchange. In view of the previously established coupling of entry of a recombinase at a cos cut and initiation of DNA packaging by that cos cut, these results imply that the recombinase (presumably the RecBC gene product) enters lambda's chromosome at the right end.
Volume 113(2)
Pages 215-27
Published 1986-6-1
PMID 3013720
PMC PMC1202835
MeSH Bacteriophage lambda / drug effects Bacteriophage lambda / genetics* DNA Restriction Enzymes DNA, Viral / metabolism* Escherichia coli / genetics Escherichia coli Proteins* Exodeoxyribonuclease V Exodeoxyribonucleases / metabolism* Phenotype Species Specificity Transduction, Genetic Trimethoprim / pharmacology
IF 3.564
Times Cited 63
Prokaryotes E. coli ME9019