RRC ID 29845
著者 Wada M, Kano Y, Ogawa T, Okazaki T, Imamoto F.
タイトル Construction and characterization of the deletion mutant of hupA and hupB genes in Escherichia coli.
ジャーナル J Mol Biol
Abstract Insertion and deletion mutations of the hupB and hupA genes, which encode the HU-1 and HU-2 proteins, respectively, of Escherichia coli, have been constructed in vitro and transferred to the hup loci on the bacterial chromosome. The mutations were constructed by inserting a gene encoding chloramphenicol resistance or kanamycin resistance into the coding region of the hupB or hupA gene, respectively. A complete deletion of the hupA gene was constructed by replacing the entire hupA coding region with the kanamycin resistance gene. Cells in which either the hupB or the hupA gene is defective grow normally, but cells in which both of the hup genes are defective exhibit phenotypes different from the wildtype strain. The hupA-hupB double mutants are cold-sensitive, although their growth rate is normal at 37 degrees C. Furthermore, the viability of the hupA-hupB double mutants is severely reduced when the cells are subjected to either cold shock or heat shock, indicating that the hup genes are essential for cell survival under some conditions of stress. The double mutants also exhibit filamentation when grown in the lower range of permissive growth temperature.
巻・号 204(3)
ページ 581-91
公開日 1988-12-5
DOI 10.1016/0022-2836(88)90357-9
PII 0022-2836(88)90357-9
PMID 3066907
MeSH Alleles Bacterial Proteins / biosynthesis Bacterial Proteins / genetics* Blotting, Southern Chromosome Deletion* Cloning, Molecular DNA-Binding Proteins / genetics* Escherichia coli / cytology Escherichia coli / genetics* Genes, Bacterial Mutation Transduction, Genetic
IF 4.76
引用数 145
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME7828