| RRC ID |
29849
|
| 著者 |
Benner D, Müller N, Boos W.
|
| タイトル |
Temperature-sensitive catabolite activator protein in Escherichia coli BUG6.
|
| ジャーナル |
J Bacteriol
|
| Abstract |
BUG6 is a temperature-sensitive cell division mutant which forms filaments at the nonpermissive temperature. Synthesis of the maltose- and galactose-binding protein-dependent transport systems is also temperature sensitive in BUG6. Using operon and protein fusions of the maltose transport genes to lacZ, we observed that the temperature-sensitive control of the maltose transport system in BUG6 occurs at the transcriptional level. By P1-mediated transductions, we found that BUG6 contains two independent temperature-sensitive mutations. One maps between 2 and 3 min on the Escherichia coli linkage map, in close proximity to the fts-envA region. This mutation is responsible for temperature-sensitive cell division. The other mutation maps at 73 min in crp, the structural gene of the catabolite activator protein. The latter could be complemented by a hybrid plasmid carrying the wild-type crp as the only gene on a 0.9-kilobase HindIII-AluI restriction fragment. The mutation in crp alone was found to be responsible for the temperature-sensitive synthesis of the maltose transport system. Although it causes a complete block of transcription of the maltose transport genes at 41 degrees C, this mutation had only a marginal effect on the transcription of the lac operon.
|
| 巻・号 |
161(1)
|
| ページ |
347-52
|
| 公開日 |
1985-1-1
|
| DOI |
10.1128/jb.161.1.347-352.1985
|
| PMID |
3155717
|
| PMC |
PMC214878
|
| MeSH |
ATP-Binding Cassette Transporters*
Bacteriophage lambda / genetics
Carrier Proteins / genetics
Cell Division
Escherichia coli / genetics*
Escherichia coli Proteins*
Lac Operon
Maltose-Binding Proteins
Monosaccharide Transport Proteins*
Mutation*
Receptors, Cyclic AMP / genetics*
Temperature
Transcription, Genetic
Transduction, Genetic
Transformation, Bacterial
|
| IF |
3.006
|
| 引用数 |
15
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME8460
ME8461
ME8462
ME8463
ME8465 |
