RRC ID 29849
Author Benner D, Müller N, Boos W.
Title Temperature-sensitive catabolite activator protein in Escherichia coli BUG6.
Journal J Bacteriol
Abstract BUG6 is a temperature-sensitive cell division mutant which forms filaments at the nonpermissive temperature. Synthesis of the maltose- and galactose-binding protein-dependent transport systems is also temperature sensitive in BUG6. Using operon and protein fusions of the maltose transport genes to lacZ, we observed that the temperature-sensitive control of the maltose transport system in BUG6 occurs at the transcriptional level. By P1-mediated transductions, we found that BUG6 contains two independent temperature-sensitive mutations. One maps between 2 and 3 min on the Escherichia coli linkage map, in close proximity to the fts-envA region. This mutation is responsible for temperature-sensitive cell division. The other mutation maps at 73 min in crp, the structural gene of the catabolite activator protein. The latter could be complemented by a hybrid plasmid carrying the wild-type crp as the only gene on a 0.9-kilobase HindIII-AluI restriction fragment. The mutation in crp alone was found to be responsible for the temperature-sensitive synthesis of the maltose transport system. Although it causes a complete block of transcription of the maltose transport genes at 41 degrees C, this mutation had only a marginal effect on the transcription of the lac operon.
Volume 161(1)
Pages 347-52
Published 1985-1-1
DOI 10.1128/JB.161.1.347-352.1985
PMID 3155717
PMC PMC214878
MeSH ATP-Binding Cassette Transporters* Bacteriophage lambda / genetics Carrier Proteins / genetics Cell Division Escherichia coli / genetics* Escherichia coli Proteins* Lac Operon Maltose-Binding Proteins Monosaccharide Transport Proteins* Mutation* Receptors, Cyclic AMP / genetics* Temperature Transcription, Genetic Transduction, Genetic Transformation, Bacterial
IF 3.234
Times Cited 15
Prokaryotes E. coli ME8460 ME8461 ME8462 ME8463 ME8465