RRC ID 29870
Author Sullivan MA, Bock RM.
Title Isolation and characterization of antisuppressor mutations in Escherichia coli.
Journal J Bacteriol
Abstract Nonsense mutations in lacI have been shown to be useful as indicators of the efficiency of nonsense suppression. From strains containing supE and a lacI nonsense mutation, selection for LacI- mutants has resulted in the isolation of four antisuppressor mutations. Tn10 insertions linked to these mutations were isolated and used to group the four mutations into three loci. The asuA1 and asuA2 mutations are linked to trp, reduce suppression by supE approximately twofold, and affect a variety of suppressors. The asuB3 mutation was mapped by P1 cotransduction to rpsL but does not confer resistance to streptomycin. The asuC4 mutation reduced suppression by supE by 95% and was shown biochemically to result in the loss of two pseudouridine modifications from the 3' side of the anticodon stem and loop of tRNA2Gln. This mutation is linked to purF, suggesting that it is a new allele of hisT.
Volume 161(1)
Pages 377-84
Published 1985-1-1
DOI 10.1128/jb.161.1.377-384.1985
PMID 3918006
PMC PMC214882
MeSH Base Sequence Codon Escherichia coli / genetics* Escherichia coli Proteins Genes, Bacterial Lac Operon Mutation* Pseudouridine RNA, Transfer / genetics RNA, Transfer, Amino Acyl / genetics Ribosomal Protein S9 Suppression, Genetic* Transduction, Genetic beta-Galactosidase / biosynthesis beta-Galactosidase / genetics
IF 3.006
Times Cited 10
Prokaryotes E. coli ME8386 ME8387 ME8388 ME8389 ME8390 ME8391