論文 - 詳細
RRC ID | 29892 |
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著者 | Lugtenberg EJ, v Schijndel-van Dam A. |
タイトル | Temperature-sensitive mutants of Escherichia coli K-12 with low activities of the L-alanine adding enzyme and the D-alanyl-D-alanine adding enzyme. |
ジャーナル | J Bacteriol |
Abstract |
A number of properties of temperature-sensitive mutants in murein synthesis are described. The mutants grow at 30 C but lyse at 42 C. One mutant possesses a temperature-sensitive d-alanyl-d-alanine adding enzyme, has an impaired rate of murein synthesis in vivo at both 30 and 42 C, and contains elevated levels of uridine diphosphate-N-acetyl-muramyl-tripeptide (UDP-MurNAc-l-Ala-d-Glu-m-diaminopimelic acid) at 42 C. The other mutant possesses an l-alanine adding enzyme with a very low in vitro activity at both 30 and 42 C. Its in vivo rate of murein synthesis is almost normal at 30 C but is much less at 42 C. When the murein precursors were isolated after incubation of the cells in the presence of (14)C-l-alanine, they contained only a fraction of the radioactivity that could be obtained from a wild-type strain. A genetic nomenclature for genes concerned with murein synthesis is proposed. |
巻・号 | 110(1) |
ページ | 35-40 |
公開日 | 1972-4-1 |
DOI | 10.1128/jb.110.1.35-40.1972 |
PMID | 4552998 |
PMC | PMC247375 |
MeSH | Acetates Alanine / metabolism Carbon Isotopes Chloramphenicol / pharmacology Dipeptides Escherichia coli / drug effects Escherichia coli / enzymology* Escherichia coli / growth & development Escherichia coli / metabolism Genes Genetics, Microbial Glucosamine / biosynthesis Ligases* Mutation Nucleoside Diphosphate Sugars / biosynthesis Peptide Biosynthesis Peptidoglycan / biosynthesis Temperature Time Factors Uracil Nucleotides |
IF | 3.006 |
リソース情報 | |
原核生物(大腸菌) | JE6608 JE6606 |