RRC ID 29892
著者 Lugtenberg EJ, v Schijndel-van Dam A.
タイトル Temperature-sensitive mutants of Escherichia coli K-12 with low activities of the L-alanine adding enzyme and the D-alanyl-D-alanine adding enzyme.
ジャーナル J Bacteriol
Abstract A number of properties of temperature-sensitive mutants in murein synthesis are described. The mutants grow at 30 C but lyse at 42 C. One mutant possesses a temperature-sensitive d-alanyl-d-alanine adding enzyme, has an impaired rate of murein synthesis in vivo at both 30 and 42 C, and contains elevated levels of uridine diphosphate-N-acetyl-muramyl-tripeptide (UDP-MurNAc-l-Ala-d-Glu-m-diaminopimelic acid) at 42 C. The other mutant possesses an l-alanine adding enzyme with a very low in vitro activity at both 30 and 42 C. Its in vivo rate of murein synthesis is almost normal at 30 C but is much less at 42 C. When the murein precursors were isolated after incubation of the cells in the presence of (14)C-l-alanine, they contained only a fraction of the radioactivity that could be obtained from a wild-type strain. A genetic nomenclature for genes concerned with murein synthesis is proposed.
巻・号 110(1)
ページ 35-40
公開日 1972-4-1
DOI 10.1128/jb.110.1.35-40.1972
PMID 4552998
PMC PMC247375
MeSH Acetates Alanine / metabolism Carbon Isotopes Chloramphenicol / pharmacology Dipeptides Escherichia coli / drug effects Escherichia coli / enzymology* Escherichia coli / growth & development Escherichia coli / metabolism Genes Genetics, Microbial Glucosamine / biosynthesis Ligases* Mutation Nucleoside Diphosphate Sugars / biosynthesis Peptide Biosynthesis Peptidoglycan / biosynthesis Temperature Time Factors Uracil Nucleotides
IF 3.006
リソース情報
原核生物(大腸菌) JE6608 JE6606