論文 - 詳細
| RRC ID | 29893 |
|---|---|
| 著者 | Lugtenberg EJ, v Schijndel-van Dam A. |
| タイトル | Temperature-sensitive mutants of Escherichia coli K-12 with low activity of the diaminopimelic acid adding enzyme. |
| ジャーナル | J Bacteriol |
| Abstract |
Five temperature-sensitive lysis mutants were found to possess very low diaminopimelic acid (Dpm) adding enzyme activity in vitro. Murein synthesis at 42 C was impaired, and uridine-5'-diphosphate-N-acetyl-muramyl-l-Ala- d-Glu (UDP-MurNAc-dipeptide) was accumulated. In the presence of NaCl, the mutants could grow at 42 C. NaCl had no influence on the Dpm adding enzyme activity in vitro. The growth rate of most temperature-resistant revertants was decreased, but their Dpm adding enzyme activity remained very low. Two revertants had a rather normal growth rate. Their Dpm adding enzyme activity was significantly increased, but much lower than in the wild type. The influence of growth rate on the viability of the mutants is discussed. |
| 巻・号 | 110(1) |
| ページ | 41-6 |
| 公開日 | 1972-4-1 |
| DOI | 10.1128/jb.110.1.41-46.1972 |
| PMID | 4553000 |
| PMC | PMC247376 |
| MeSH | Alanine / metabolism Amino Acids Carbon Isotopes Dipeptides Escherichia coli / drug effects Escherichia coli / enzymology* Escherichia coli / growth & development Escherichia coli / metabolism Genes Genetics, Microbial Glucosamine Glutamates Ligases* Mutation Nucleoside Diphosphate Sugars Peptides Peptidoglycan / biosynthesis Pimelic Acids Sodium Chloride / pharmacology Sucrose / pharmacology Temperature Time Factors Uracil Nucleotides |
| IF | 3.006 |
| リソース情報 | |
| 原核生物(大腸菌) | JE6604 |