RRC ID 299
Author Kamiyama S, Suda T, Ueda R, Suzuki M, Okubo R, Kikuchi N, Chiba Y, Goto S, Toyoda H, Saigo K, Watanabe M, Narimatsu H, Jigami Y, Nishihara S.
Title Molecular cloning and identification of 3'-phosphoadenosine 5'-phosphosulfate transporter.
Journal J. Biol. Chem.
Abstract Nucleotide sulfate, namely 3'-phosphoadenosine 5'-phosphosulfate (PAPS), is a universal sulfuryl donor for sulfation. Although a specific PAPS transporter is present in Golgi membrane, no study has reported the corresponding gene. We have identified a novel human gene encoding a PAPS transporter, which we have named PAPST1, and the Drosophila melanogaster ortholog, slalom (sll). The amino acid sequence of PAPST1 (432 amino acids) exhibited 48.1% identity with SLL (465 amino acids), and hydropathy analysis predicted the two to be type III transmembrane proteins. The transient expression of PAPST1 in SW480 cells showed a subcellular localization in Golgi membrane. The expression of PAPST1 and SLL in yeast Saccharomyces cerevisiae significantly increased the transport of PAPS into the Golgi membrane fraction. In human tissues, PAPST1 is highly expressed in the placenta and pancreas and present at lower levels in the colon and heart. An RNA interference fly of sll produced with a GAL4-UAS system revealed that the PAPS transporter is essential for viability. It is well known that mutations of some genes related to PAPS synthesis are responsible for human inherited disorders. Our findings provide insights into the significance of PAPS transport and post-translational sulfation.
Volume 278(28)
Pages 25958-63
Published 2003-7-11
DOI 10.1074/jbc.M302439200
PII M302439200
PMID 12716889
MeSH Amino Acid Sequence Animals Biological Transport Blotting, Western Cell Membrane / metabolism Cloning, Molecular Colon / metabolism DNA, Complementary / metabolism Dose-Response Relationship, Drug Drosophila Proteins / chemistry Drosophila melanogaster Golgi Apparatus / metabolism Humans Membrane Transport Proteins / chemistry* Membrane Transport Proteins / genetics* Microscopy, Fluorescence Molecular Sequence Data Myocardium / metabolism Pancreas / metabolism Phosphoadenosine Phosphosulfate / metabolism* Phylogeny Plasmids / metabolism Protein Processing, Post-Translational RNA Interference RNA, Double-Stranded / metabolism Reverse Transcriptase Polymerase Chain Reaction Saccharomyces cerevisiae / metabolism Sequence Homology, Amino Acid Subcellular Fractions Substrate Specificity Sulfate Transporters Tissue Distribution Transfection
IF 4.106
Times Cited 66