Reference - Detail
| RRC ID | 29900 |
|---|---|
| Author | Miyakawa T, Matsuzawa H, Matsuhashi M, Sugino Y. |
| Title | Cell wall peptidoglycan mutants of Escherichia coli K-12: existence of two clusters of genes, mra and mrb, for cell wall peptidoglycan biosynthesis. |
| Journal | J Bacteriol |
| Abstract |
Temperature-sensitive mutants of Escherichia coli K-12 which cannot form cell wall peptidoglycan at 42 C were isolated. The thermosensitive steps were characterized biochemically, and the genes coding the enzymes of peptidoglycan synthesis were mapped. These genes were in two clusters: one group, located at about 1.5 min between leu and azi, was designated as mra (murein a); the second group, located at about 77.5 min close to argH and metB, was designated as mrb (murein b, with the order metB-argH-mrb). No simple relations were found between the gene location and the order or localization of enzymes involved in the sequence of reactions of cell wall peptidoglycan biosynthesis. |
| Volume | 112(2) |
| Pages | 950-8 |
| Published | 1972-11-1 |
| DOI | 10.1128/jb.112.2.950-958.1972 |
| PMID | 4563986 |
| PMC | PMC251507 |
| MeSH | Alanine / metabolism Carbon Isotopes Carboxypeptidases / metabolism Cell Wall / metabolism* Cell-Free System Chromatography, Paper Chromosome Mapping* Chromosomes, Bacterial* Escherichia coli / enzymology Escherichia coli / isolation & purification Escherichia coli / metabolism* Glucosamine / metabolism Ligases / metabolism Mutation* Oxidoreductases / metabolism Peptide Synthases / metabolism Peptidoglycan / biosynthesis* Phosphoenolpyruvate Stereoisomerism Temperature Transduction, Genetic Transferases / metabolism Uridine / metabolism |
| IF | 3.006 |
| Resource | |
| Prokaryotes E. coli | JE6614 JE6610 JE6612 |