Reference - Detail
|Author||Bukhari AI, Taylor AL.|
|Title||Genetic analysis of diaminopimelic acid- and lysine-requiring mutants of Escherichia coli.|
Several diaminopimelic acid (DAP)- and lysine-requiring mutants of Escherichia coli were isolated and studied by genetic, physiological, and biochemical means. The genes concerned with DAP-lysine synthesis map at several different sites on the E. coli chromosome and, therefore, do not constitute a single operon. Three separate loci affecting DAP synthesis are located in the 0 to 2.5 min region of the genetic map. The order of the loci in this region is thr-dapB-pyrA-ara-leu-pan-dapC-tonA-dapD. Two additional DAP genes map in the region between min 47 and 48, with the gene order being gua-dapA-dapE-ctr. The lys locus at min 55 determines the synthesis of the enzyme DAP decarboxylase, which catalyzes the conversion of DAP into lysine. The order of the genes in this region is serA-lysA-thyA.
|MeSH||Carboxy-Lyases / biosynthesis Carboxy-Lyases / metabolism Cell-Free System Chromatography, Paper Chromosome Mapping* Colorimetry Conjugation, Genetic Culture Media Escherichia coli / enzymology Escherichia coli / growth & development Escherichia coli / isolation & purification Escherichia coli / metabolism* Genes Genetics, Microbial* Isomerases / metabolism Lysine / biosynthesis Lysine / metabolism* Mutation* Pimelic Acids / biosynthesis Pimelic Acids / metabolism* Transduction, Genetic|
|Prokaryotes E. coli||ME5458 ME8560 ME8728|