RRC ID 30019
著者 Loewen PC, Triggs BL.
タイトル Genetic mapping of katF, a locus that with katE affects the synthesis of a second catalase species in Escherichia coli.
ジャーナル J Bacteriol
Abstract A class of catalase-deficient mutants that was unlinked to katE was localized between mutS and cys at 59.0 min on the Escherichia coli genome. This locus was named katF. Transposon Tn10 insertions were isolated that mapped in both katE and katF loci. The catalase species present in katE+ and katF+ recombinants was found to be different from the main catalase activities, HPI and HPII, in several respects. It did not have an associated peroxidase activity; it was electrophoretically slower on native polyacrylamide gels; it eluted from DEAE-Sephadex A50 at a higher salt concentration; its Km for H2O2 was 30.9 mM as compared with 3.7 mM for HPI and HPII; its synthesis was not induced by ascorbate; and it did not cross react with HPI-HPII antisera. This new catalase was labeled HPIII.
巻・号 160(2)
ページ 668-75
公開日 1984-11-1
DOI 10.1128/jb.160.2.668-675.1984
PMID 6094482
PMC PMC214787
MeSH Ascorbic Acid / pharmacology Catalase / genetics* Catalase / metabolism Chromosome Mapping DNA Restriction Enzymes DNA Transposable Elements Escherichia coli / enzymology Escherichia coli / genetics* Genes* Genes, Bacterial* Genetic Markers* Genotype Immunodiffusion Kinetics Species Specificity
IF 3.006
引用数 169
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME8300 ME8301 ME8302