RRC ID 30029
著者 Masters PS, Hong JS.
タイトル Genetics of the glutamine transport system in Escherichia coli.
ジャーナル J Bacteriol
Abstract The active transport of glutamine by Escherichia coli occurs via a single osmotic shock-sensitive transport system which is known to be dependent upon a periplasmic binding protein specific for glutamine. We obtained a mutant that had elevated levels of glutamine transport and overproduced the glutamine binding protein. From this strain many point mutants and deletion-carrying strains defective in glutamine transport were isolated by a variety of techniques. The genetic locus coding for the glutamine transport system, glnP, and the regulatory mutation which causes overproduction of the transport system were both shown to map at 17.7 min on the E. coli chromosome, and it was demonstrated that the glnP locus contains the structural gene for the glutamine binding protein. Evidence was also obtained that the glutamine transport system, by an unknown mechanism, plays a direct role in the catabolism of glutamate and, hence, of glutamine and proline as well.
巻・号 147(3)
ページ 805-19
公開日 1981-9-1
DOI 10.1128/jb.147.3.805-819.1981
PMID 6115851
PMC PMC216116
MeSH Biological Transport, Active Carrier Proteins / genetics Chromosome Mapping Chromosomes, Bacterial Escherichia coli / genetics* Escherichia coli / metabolism Genes Genes, Bacterial* Genes, Regulator Glutamates / metabolism Glutamic Acid Glutamine / metabolism* Mutation
IF 3.006
引用数 40
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME8692