RRC ID 30035
Author Davis NK, Greer S, Jones-Mortimer MC, Perham RN.
Title Isolation and mapping of glutathione reductase-negative mutants of Escherichia coli K12.
Journal J Gen Microbiol
Abstract Two independent mutants defective in glutathione reductase (EC 1.6.4.2) were isolated in an Escherichia coli K12 strain lysogenized with bacteriophage Mu. The prophage was lost (and the ability to reduce glutathione regained) by 32% of the xylose-positive transductants when T4GT7 was used as the vector, but the markers were not cotransduced by P1. Similarly, the prophage site and malA were cotransduced by T4GT7 but not by P1. The gor gene maps between min 77 and 78 on the E. coli genome, and the mutation causes no growth defect.
Volume 128(7)
Pages 1631-4
Published 1982-7-1
DOI 10.1099/00221287-128-7-1631
PMID 6214613
MeSH Bacteriophage mu / genetics Chromosome Mapping Chromosomes, Bacterial Escherichia coli / enzymology Escherichia coli / genetics* Glutathione Reductase / metabolism* Lysogeny Mutation Transduction, Genetic
Times Cited 36
WOS Category MICROBIOLOGY
Resource
Prokaryotes E. coli ME8609