論文 - 詳細
| RRC ID | 30038 |
|---|---|
| 著者 | Sadler JR, Tecklenburg M, Betz JL. |
| タイトル | Plasmids containing many tandem copies of a synthetic lactose operator. |
| ジャーナル | Gene |
| Abstract |
Up to 12 tandem copies of the lactose operator sequence AATTCCACATGTGGAATTGTGAGCGGATAACAATTTGTGG (3') GGTGTACACCTTAACACTCGCCTATTGTTAAACACCTTAA (5') have been cloned in the EcoRI site of plasmid pMB9. A 12-operator plasmid is about 8% operator by weight and represents a rich source of this DNA segment. A procedure for the rapid and convenient isolation of operator in mg quantities is presented. The lifetimes of complexes formed between repressor and oligo-operator plasmids increased with increasing numbers of tandem operators per plasmid. Evidence is presented indicating that only one tetrameric repressor molecule binds strongly to a segment of four (or fewer) tandem operators, but that two repressor molecules can be accommodated on segments containing at least six tandem operators. |
| 巻・号 | 8(3) |
| ページ | 279-300 |
| 公開日 | 1980-2-1 |
| DOI | 10.1016/0378-1119(80)90005-0 |
| PII | 0378-1119(80)90005-0 |
| PMID | 6244215 |
| MeSH | Base Sequence DNA Restriction Enzymes / metabolism DNA, Recombinant / analysis* Electrophoresis Escherichia coli / genetics Lac Operon* Plasmids* |
| IF | 2.984 |
| 引用数 | 150 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 31 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME8134 ME8135 ME8136 ME8133 |