RRC ID 30038
著者 Sadler JR, Tecklenburg M, Betz JL.
タイトル Plasmids containing many tandem copies of a synthetic lactose operator.
ジャーナル Gene
Abstract Up to 12 tandem copies of the lactose operator sequence AATTCCACATGTGGAATTGTGAGCGGATAACAATTTGTGG (3') GGTGTACACCTTAACACTCGCCTATTGTTAAACACCTTAA (5') have been cloned in the EcoRI site of plasmid pMB9. A 12-operator plasmid is about 8% operator by weight and represents a rich source of this DNA segment. A procedure for the rapid and convenient isolation of operator in mg quantities is presented. The lifetimes of complexes formed between repressor and oligo-operator plasmids increased with increasing numbers of tandem operators per plasmid. Evidence is presented indicating that only one tetrameric repressor molecule binds strongly to a segment of four (or fewer) tandem operators, but that two repressor molecules can be accommodated on segments containing at least six tandem operators.
巻・号 8(3)
ページ 279-300
公開日 1980-2-1
DOI 10.1016/0378-1119(80)90005-0
PII 0378-1119(80)90005-0
PMID 6244215
MeSH Base Sequence DNA Restriction Enzymes / metabolism DNA, Recombinant / analysis* Electrophoresis Escherichia coli / genetics Lac Operon* Plasmids*
IF 2.984
引用数 150
WOS 分野 GENETICS & HEREDITY
リソース情報
原核生物(大腸菌) ME8134 ME8135 ME8136 ME8133