RRC ID 30042
Author Fouts KE, Barbour SD.
Title Insertion of transposons through the major cotransduction gap of Escherichia coli K-12.
Journal J. Bacteriol.
Abstract The major cotransduction gap of the Escherichia coli chromosome extends from mini 31 to 34. We have inserted transposons through this gap which, by sequential transduction, link sbcA (min 29.8) with manA (min 35.7) and thus eliminate the gap. These results indicate that the length of DNA in the region, as measured by transduction, is not significantly different from the length obtained by conjugational time of entry. Since this segment of the E. coli chromosome has few known genes, these transposon insertions will be useful for genetic manipulations in the region of the gap. We describe the usefulness of these markers for rapidly mapping mutations which may be isolated in the region from min 27 to 37.
Volume 149(1)
Pages 106-13
Published 1982-1
PMID 6274840
PMC PMC216597
MeSH Chromosome Mapping Chromosomes, Bacterial* DNA Transposable Elements* Escherichia coli / genetics* Genetic Linkage Genetic Markers* Mutation Transduction, Genetic*
IF 3.234
Times Cited 26
WOS Category MICROBIOLOGY
Resource
Prokaryotes E. coli ME8708 ME8582 ME8583 ME8584 ME8585 ME8586 ME8587 ME8588 ME8589