Reference - Detail
RRC ID | 30053 |
---|---|
Author | Brooks JE, Blumenthal RM, Gingeras TR. |
Title | The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene. |
Journal | Nucleic Acids Res |
Abstract |
The E. coli dam (DNA adenine methylase) enzyme is known to methylate the sequence GATC. A general method for cloning sequence-specific DNA methylase genes was used to isolate the dam gene on a 1.14 kb fragment, inserted in the plasmid vector pBR322. Subsequent restriction mapping and subcloning experiments established a set of approximate boundaries of the gene. The nucleotide sequence of the dam gene was determined, and analysis of that sequence revealed a unique open reading frame which corresponded in length to that necessary to code for a protein the size of dam. Amino acid composition derived from this sequence corresponds closely to the amino acid composition of the purified dam protein. Enzymatic and DNA:DNA hybridization methods were used to investigate the possible presence of dam genes in a variety of prokaryotic organisms. |
Volume | 11(3) |
Pages | 837-51 |
Published | 1983-2-11 |
DOI | 10.1093/nar/11.3.837 |
PMID | 6300769 |
PMC | PMC325756 |
MeSH | Amino Acid Sequence Base Sequence Cloning, Molecular* DNA (Cytosine-5-)-Methyltransferases / genetics* DNA Restriction Enzymes DNA, Bacterial / genetics DNA, Bacterial / isolation & purification* Escherichia coli / enzymology* Genes* Genes, Bacterial* Methyltransferases / genetics* Nucleic Acid Hybridization Plasmids |
IF | 11.502 |
Times Cited | 152 |
WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Prokaryotes E. coli | ME9000 |