RRC ID 30055
Author Elledge SJ, Walker GC.
Title Proteins required for ultraviolet light and chemical mutagenesis. Identification of the products of the umuC locus of Escherichia coli.
Journal J. Mol. Biol.
Abstract umuC is the genetic locus showing the greatest specificity for the "error-prone repair" process that is responsible for u.v. and chemical mutagenesis in Escherichia coli. By generating a probe specific to umuC DNA, we have been able to clone the umuC locus. Through a combination of subcloning and Tn1000 mutagenesis, we have identified a region of 2.2 X 10(3) bases which contains the information necessary to complement umuC mutations. This region of DNA codes for two polypeptides with molecular weights of 16,000 and 45,000. The genes for these proteins are organized in an operon that is repressed by the lexA protein. Complementation of previously isolated umuC mutations revealed that these proteins correspond to two complementation groups, umuC, which codes for the 45,000 Mr protein, and umuD, which codes for the 16,000 Mr protein, and that therefore both proteins are essential for "error-prone repair" in E. coli.
Volume 164(2)
Pages 175-92
Published 1983-2-25
PII 0022-2836(83)90074-8
PMID 6302271
MeSH Bacterial Proteins / biosynthesis Bacterial Proteins / genetics* Chromosome Mapping Chromosomes, Bacterial DNA Repair DNA Transposable Elements Escherichia coli / genetics* Escherichia coli / metabolism Escherichia coli / radiation effects Genetic Complementation Test Mutation* Plasmids Repressor Proteins / metabolism Transcription, Genetic Ultraviolet Rays*
IF 5.067
Times Cited 254
Prokaryotes E. coli ME8412 ME8415