RRC ID 30058
Author Lorence MC, Rupert CS.
Title Convenient construction of recA deletion derivatives of Escherichia coli.
Journal J Bacteriol
Abstract Two Escherichia coli K-12 Hfr strains have been constructed which transfer a recA deletion, which is highly linked to a Tn10 insertion conferring tetracycline resistance, early during conjugation. These strains transfer the recA deletion in opposite directions with different origins of transfer, allowing for preservation of desirable recipient strain markers either clockwise or counterclockwise of recA.
Volume 156(1)
Pages 458-9
Published 1983-10-1
DOI 10.1128/jb.156.1.458-459.1983
PMID 6311804
PMC PMC215109
MeSH Chromosome Deletion* Conjugation, Genetic DNA Transposable Elements Escherichia coli / genetics* Genes, Bacterial* Mutation Rec A Recombinases* / genetics*
IF 3.006
Times Cited 18
WOS Category MICROBIOLOGY
Resource
Prokaryotes E. coli ME8271 ME8272