RRC ID 30117
著者 Nishimura K, Nakayashiki T, Inokuchi H.
タイトル Cloning and identification of the hemG gene encoding protoporphyrinogen oxidase (PPO) of Escherichia coli K-12.
ジャーナル DNA Res
Abstract Cells of the VSR751 strain, which was previously isolated as a photoresistant revertant of the visA-deleted (hemH-deleted) strain of Escherichia coli K-12, accumulated uroporphyrin (uro), coproporphyrin (copro) and protoporphyrin IX (proto), but did not accumulate as much protoporphyrin as cells of the parental strain (hemH-deleted). Therefore, we concluded that strain VSR751 must be defective in protoporphyrinogen oxidase (PPO), the product of the hemG gene. By complementation analysis using VSR751, we isolated and identified this gene. The hemG gene is located at 86 mim on the E. coli chromosome, just upstream of the rrnA operon, and is transcribed clockwise in the same direction as the rrnA operon. This gene encodes a 181-amino acid protein with a calculated molecular mass of about 21 kDa. Sequence analysis revealed the presence of flavodoxin motif, suggesting tha a cofactor of this enzyme is flavin mononucleotide, which is consistent with the previous report that the mammalian PPO had the flavin cofactor.
巻・号 2(1)
ページ 1-8
公開日 1995-1-1
DOI 10.1093/dnares/2.1.1
PMID 7788523
MeSH Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Bacterial Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli Proteins Genetic Complementation Test Molecular Sequence Data Open Reading Frames Oxidoreductases / genetics* Oxidoreductases Acting on CH-CH Group Donors* Protoporphyrinogen Oxidase
IF 4.009
リソース情報
原核生物(大腸菌) IH75 IH76