論文 - 詳細
| RRC ID | 30118 |
|---|---|
| 著者 | Cao Y, Kogoma T. |
| タイトル | The mechanism of recA polA lethality: suppression by RecA-independent recombination repair activated by the lexA(Def) mutation in Escherichia coli. |
| ジャーナル | Genetics |
| Abstract |
The mechanism of recA polA lethality in Escherichia coli has been studied. Complementation tests have indicated that both the 5'-->3' exonuclease and the polymerization activities of DNA polymerase I are essential for viability in the absence of RecA protein, whereas the viability and DNA replication of DNA polymerase I-defective cells depend on the recombinase activity of RecA. An alkaline sucrose gradient sedimentation analysis has indicated that RecA has only a minor role in Okazaki fragment processing. Double-strand break repair is proposed for the major role of RecA in the absence of DNA polymerase I. The lexA(Def)::Tn5 mutation has previously been shown to suppress the temperature-sensitive growth of recA200(Ts) polA25::spc mutants. The lexA(Def) mutation can alleviate impaired DNA synthesis in the recA200(Ts) polA25::spc mutant cells at the restrictive temperature. recF+ is essential for this suppression pathway. recJ and recQ mutations have minor but significant adverse effects on the suppression. The recA200(Ts) allele in the recA200(Ts) polA25::spc lexA(Def) mutant can be replaced by delta recA, indicating that the lexA(Def)-induced suppression is RecA independent. lexA(Def) reduces the sensitivity of delta recA polA25::spc cells to UV damage by approximately 10(4)-fold. lexA(Def) also restores P1 transduction proficiency to the delta recA polA25::spc mutant to a level that is 7.3% of the recA+ wild type. These results suggest that lexA(Def) activates a RecA-independent, RecF-dependent recombination repair pathway that suppresses the defect in DNA replication in recA polA double mutants. |
| 巻・号 | 139(4) |
| ページ | 1483-94 |
| 公開日 | 1995-4-1 |
| DOI | 10.1093/genetics/139.4.1483 |
| PMID | 7789754 |
| PMC | PMC1206478 |
| MeSH | Bacterial Proteins / genetics DNA Nucleotidyltransferases / metabolism DNA Polymerase I / metabolism DNA Repair* DNA Replication Escherichia coli / genetics* Genes, Lethal* Integrases* Mutation Protein Biosynthesis Rec A Recombinases / genetics Rec A Recombinases / metabolism Recombinases Recombination, Genetic* Regulon Serine Endopeptidases* Temperature |
| IF | 4.015 |
| 引用数 | 35 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 原核生物(大腸菌) | ME9565 ME9562 ME9543 ME9544 ME9545 ME9536 ME9533 ME9523 ME9503 ME9493 ME9489 ME9362 ME9485 ME9468 ME9470 ME9467 |