| RRC ID |
30134
|
| 著者 |
Kogoma T, Hong X, Cadwell GW, Barnard KG, Asai T.
|
| タイトル |
Requirement of homologous recombination functions for viability of the Escherichia coli cell that lacks RNase HI and exonuclease V activities.
|
| ジャーナル |
Biochimie
|
| Abstract |
rnhA224 and rnhA339::cat mutants which lack RNase HI activity were found to constitutively express the sfiA::lacZ operon fusion in a recA+ lexA(+)-dependent manner. The sfiA::lacZ expression (indicating SOS induction) in rnhA mutants was increased to higher levels by the introduction of the recD1903 or recB21 mutation. The SOS induction in these cells was further enhanced by nutritional shift up from casamino acid medium to Luria broth. Although the extent by which the recD and recB mutations increased the sfiA expression in rnhA mutants was similar, the rnhA224 recB21 double mutant had plating efficiencies that were 25-fold lower on casamino acid plates and 5 x 10(5)-fold lower on Luria broth plates than the respective plating efficiencies of either rnhA224 recD or rnhA::cat recD double mutants. Whereas the recD mutation inactivates the exonuclease activity of the RecBCD (Exo V) enzyme without reducing the recombination proficiency of the mutant, the recB21 mutation abolishes both the exonuclease activity and recombination capability. Therefore, in the absence of both RNase HI and Exo V activities, homologous recombination functions become crucial for viability, particularly in Luria broth. Introduction of mutations in recA, recJ and recN exacerbated the phenotypes. It is proposed that R-loops which persist due to the lack of RNase HI activity can be removed by two alternative routes of DNA repair: one involving Exo V, Exo I and DNA polymerase I, and the other involving both the RecBCD and RecF pathways of homologous recombination activities. The isolation of RNA polymerase mutants that constitutively express the SOS response at high levels and exhibit remarkable broth-sensitivity lend strong support to the contention that increased amounts of the persisting R-loop in rnhA mutants growing in Luria broth give rise to a stronger SOS response.
|
| 巻・号 |
75(1-2)
|
| ページ |
89-99
|
| 公開日 |
1993-1-1
|
| DOI |
10.1016/0300-9084(93)90029-r
|
| PII |
0300-9084(93)90029-R
|
| PMID |
8389213
|
| MeSH |
DNA Helicases / metabolism*
DNA, Bacterial / biosynthesis
DNA-Directed RNA Polymerases / genetics
Escherichia coli / enzymology*
Escherichia coli Proteins*
Exodeoxyribonuclease V
Exodeoxyribonucleases / metabolism*
Mutation*
Recombination, Genetic*
Ribonuclease H / metabolism*
SOS Response, Genetics*
|
| IF |
3.413
|
| 引用数 |
44
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
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