RRC ID |
30141
|
著者 |
Kido M, Yamanaka K, Mitani T, Niki H, Ogura T, Hiraga S.
|
タイトル |
RNase E polypeptides lacking a carboxyl-terminal half suppress a mukB mutation in Escherichia coli.
|
ジャーナル |
J Bacteriol
|
Abstract |
We have isolated suppressor mutants that suppress temperature-sensitive colony formation and anucleate cell production of a mukB mutation. A linkage group (smbB) of the suppressor mutations is located in the rne/ams/hmp gene encoding the processing endoribonuclease RNase E. All of the rne (smbB) mutants code for truncated RNase E polypeptides lacking a carboxyl-terminal half. The amount of MukB protein was higher in these rne mutants than that in the rne+ strain. These rne mutants grew nearly normally in the mukB+ genetic background. The copy number of plasmid pBR322 in these rne mutants was lower than that in the rne+ isogenic strain. The results suggest that these rne mutations increase the half-lives of mukB mRNA and RNAI of pBR322, the antisense RNA regulating ColE1-type plasmid replication. We have demonstrated that the wild-type RNase E protein bound to polynucleotide phosphorylase (PNPase) but a truncated RNase E polypeptide lacking the C-terminal half did not. We conclude that the C-terminal half of RNase E is not essential for viability but plays an important role for binding with PNPase. RNase E and PNPase of the multiprotein complex presumably cooperate for effective processing and turnover of specific substrates, such as mRNAs and other RNAs in vivo.
|
巻・号 |
178(13)
|
ページ |
3917-25
|
公開日 |
1996-7-1
|
DOI |
10.1128/jb.178.13.3917-3925.1996
|
PMID |
8682798
|
PMC |
PMC232654
|
MeSH |
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Base Sequence
Binding Sites
Chromosomal Proteins, Non-Histone*
Chromosome Mapping
Cloning, Molecular
DNA, Bacterial
Endoribonucleases / chemistry
Endoribonucleases / genetics
Endoribonucleases / metabolism*
Escherichia coli / genetics*
Escherichia coli / metabolism
Escherichia coli Proteins*
Gene Dosage
Genetic Complementation Test
Molecular Sequence Data
Peptides / chemistry
Peptides / metabolism
Plasmids
Polyribonucleotide Nucleotidyltransferase / metabolism
Suppression, Genetic*
Temperature
Transduction, Genetic
|
IF |
3.006
|
引用数 |
114
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
原核生物(大腸菌) |
ME7801
ME7802
ME7803 |