論文 - 詳細
| RRC ID | 30141 |
|---|---|
| 著者 | Kido M, Yamanaka K, Mitani T, Niki H, Ogura T, Hiraga S. |
| タイトル | RNase E polypeptides lacking a carboxyl-terminal half suppress a mukB mutation in Escherichia coli. |
| ジャーナル | J Bacteriol |
| Abstract |
We have isolated suppressor mutants that suppress temperature-sensitive colony formation and anucleate cell production of a mukB mutation. A linkage group (smbB) of the suppressor mutations is located in the rne/ams/hmp gene encoding the processing endoribonuclease RNase E. All of the rne (smbB) mutants code for truncated RNase E polypeptides lacking a carboxyl-terminal half. The amount of MukB protein was higher in these rne mutants than that in the rne+ strain. These rne mutants grew nearly normally in the mukB+ genetic background. The copy number of plasmid pBR322 in these rne mutants was lower than that in the rne+ isogenic strain. The results suggest that these rne mutations increase the half-lives of mukB mRNA and RNAI of pBR322, the antisense RNA regulating ColE1-type plasmid replication. We have demonstrated that the wild-type RNase E protein bound to polynucleotide phosphorylase (PNPase) but a truncated RNase E polypeptide lacking the C-terminal half did not. We conclude that the C-terminal half of RNase E is not essential for viability but plays an important role for binding with PNPase. RNase E and PNPase of the multiprotein complex presumably cooperate for effective processing and turnover of specific substrates, such as mRNAs and other RNAs in vivo. |
| 巻・号 | 178(13) |
| ページ | 3917-25 |
| 公開日 | 1996-7-1 |
| DOI | 10.1128/jb.178.13.3917-3925.1996 |
| PMID | 8682798 |
| PMC | PMC232654 |
| MeSH | Bacterial Proteins / genetics* Bacterial Proteins / metabolism Base Sequence Binding Sites Chromosomal Proteins, Non-Histone* Chromosome Mapping Cloning, Molecular DNA, Bacterial Endoribonucleases / chemistry Endoribonucleases / genetics Endoribonucleases / metabolism* Escherichia coli / genetics* Escherichia coli / metabolism Escherichia coli Proteins* Gene Dosage Genetic Complementation Test Molecular Sequence Data Peptides / chemistry Peptides / metabolism Plasmids Polyribonucleotide Nucleotidyltransferase / metabolism Suppression, Genetic* Temperature Transduction, Genetic |
| IF | 3.006 |
| 引用数 | 114 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME7801 ME7802 ME7803 |