RRC ID 30141
著者 Kido M, Yamanaka K, Mitani T, Niki H, Ogura T, Hiraga S.
タイトル RNase E polypeptides lacking a carboxyl-terminal half suppress a mukB mutation in Escherichia coli.
ジャーナル J Bacteriol
Abstract We have isolated suppressor mutants that suppress temperature-sensitive colony formation and anucleate cell production of a mukB mutation. A linkage group (smbB) of the suppressor mutations is located in the rne/ams/hmp gene encoding the processing endoribonuclease RNase E. All of the rne (smbB) mutants code for truncated RNase E polypeptides lacking a carboxyl-terminal half. The amount of MukB protein was higher in these rne mutants than that in the rne+ strain. These rne mutants grew nearly normally in the mukB+ genetic background. The copy number of plasmid pBR322 in these rne mutants was lower than that in the rne+ isogenic strain. The results suggest that these rne mutations increase the half-lives of mukB mRNA and RNAI of pBR322, the antisense RNA regulating ColE1-type plasmid replication. We have demonstrated that the wild-type RNase E protein bound to polynucleotide phosphorylase (PNPase) but a truncated RNase E polypeptide lacking the C-terminal half did not. We conclude that the C-terminal half of RNase E is not essential for viability but plays an important role for binding with PNPase. RNase E and PNPase of the multiprotein complex presumably cooperate for effective processing and turnover of specific substrates, such as mRNAs and other RNAs in vivo.
巻・号 178(13)
ページ 3917-25
公開日 1996-7-1
DOI 10.1128/jb.178.13.3917-3925.1996
PMID 8682798
PMC PMC232654
MeSH Bacterial Proteins / genetics* Bacterial Proteins / metabolism Base Sequence Binding Sites Chromosomal Proteins, Non-Histone* Chromosome Mapping Cloning, Molecular DNA, Bacterial Endoribonucleases / chemistry Endoribonucleases / genetics Endoribonucleases / metabolism* Escherichia coli / genetics* Escherichia coli / metabolism Escherichia coli Proteins* Gene Dosage Genetic Complementation Test Molecular Sequence Data Peptides / chemistry Peptides / metabolism Plasmids Polyribonucleotide Nucleotidyltransferase / metabolism Suppression, Genetic* Temperature Transduction, Genetic
IF 3.006
引用数 114
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME7801 ME7802 ME7803