RRC ID 30144
著者 Kogoma T, Maldonado RR.
タイトル DNA polymerase I in constitutive stable DNA replication in Escherichia coli.
ジャーナル J Bacteriol
Abstract We examined the effects of mutations in the polA (encoding DNA polymerase I) and polB (DNA polymerase II) genes on inducible and constitutive stable DNA replication (iSDR and cSDR, respectively), the two alternative DNA replication systems of Escherichia coli. The polA25::miniTn10spc mutation severely inactivated cSDR, whereas polA1 mutants exhibited a significant extent of cSDR. cSDR required both the polymerase and 5'-->3' exonuclease activities of DNA polymerase I. A similar requirement for both activities was found in replication of the pBR322 plasmid in vivo. DNA polymerase II was required neither for cSDR nor for iSDR. In addition, we found that the lethal combination of an rnhA (RNase HI) and a polA mutation could be suppressed by the lexA(Def) mutation.
巻・号 179(7)
ページ 2109-15
公開日 1997-4-1
DOI 10.1128/jb.179.7.2109-2115.1997
PMID 9079893
PMC PMC178944
MeSH Bacterial Proteins / physiology DNA Polymerase I / metabolism* DNA Polymerase II / metabolism DNA Repair DNA Replication* DNA, Bacterial / biosynthesis DNA, Bacterial / radiation effects Escherichia coli / enzymology* Exodeoxyribonucleases / metabolism Mutation Plasmids Serine Endopeptidases / physiology Structure-Activity Relationship Ultraviolet Rays
IF 3.006
引用数 13
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME9613 ME9614 ME9615 ME9616 ME9617 ME9619 ME9606 ME9607 ME9608 ME9609 ME9611 ME9612 ME9600 ME9601 ME9602 ME9603 ME9604 ME9605 ME9598 ME9599 ME9505 ME9227 ME9168 ME9137