論文 - 詳細
| RRC ID | 30146 |
|---|---|
| 著者 | Bates DB, Boye E, Asai T, Kogoma T. |
| タイトル | The absence of effect of gid or mioC transcription on the initiation of chromosomal replication in Escherichia coli. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Despite the widely accepted view that transcription of gid and mioC is required for efficient initiation of cloned oriC, we show that these transcriptions have very little effect on initiation of chromosome replication at wild-type chromosomal oriC. Furthermore, neither gid nor mioC transcription is required in cells deficient in the histone-like proteins Fis or IHF. However, oriC that is sufficiently impaired for initiation by deletion of DnaA box R4 requires transcription of at least one of these genes. We conclude that transcription of mioC and especially gid is needed to activate oriC only under suboptimal conditions. We suggest that either the rifampicin-sensitive step of initiation is some other transcription occurring from promoter(s) within oriC, or the original inference of transcriptional activation derived from the rifampicin experiments is incorrect. |
| 巻・号 | 94(23) |
| ページ | 12497-502 |
| 公開日 | 1997-11-11 |
| DOI | 10.1073/pnas.94.23.12497 |
| PMID | 9356478 |
| PMC | PMC25015 |
| MeSH | Bacterial Proteins / genetics* Base Sequence Chromosomes, Bacterial / genetics* DNA Replication* DNA, Bacterial / genetics Escherichia coli / genetics* Escherichia coli Proteins* Flavoproteins* Genes, Bacterial* Molecular Sequence Data Plasmids Transcription, Genetic |
| IF | 9.412 |
| 引用数 | 33 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9391 ME9552 |