論文 - 詳細
| RRC ID | 30150 |
|---|---|
| 著者 | Yasuda T, Morimatsu K, Horii T, Nagata T, Ohmori H. |
| タイトル | Inhibition of Escherichia coli RecA coprotease activities by DinI. |
| ジャーナル | EMBO J |
| Abstract |
In Escherichia coli, the SOS response is induced upon DNA damage and results in the enhanced expression of a set of genes involved in DNA repair and other functions. The initial step, self-cleavage of the LexA repressor, is promoted by the RecA protein which is activated upon binding to single-stranded DNA. In this work, induction of the SOS response by the addition of mitomycin C was found to be prevented by overexpression of the dinI gene. dinI is an SOS gene which maps at 24.6 min of the E.coli chromosome and encodes a small protein of 81 amino acids. Immunoblotting analysis with anti-LexA antibodies revealed that LexA did not undergo cleavage in dinI-overexpressed cells after UV irradiation. In addition, the RecA-dependent conversion of UmuD to UmuD' (the active form for mutagenesis) was also inhibited in dinI-overexpressed cells. Conversely, a dinI-deficient mutant showed a slightly faster and more extensive processing of UmuD and hence higher mutability than the wild-type. Finally, we demonstrated, by using an in vitro reaction with purified proteins, that DinI directly inhibits the ability of RecA to mediate self-cleavage of UmuD. |
| 巻・号 | 17(11) |
| ページ | 3207-16 |
| 公開日 | 1998-6-1 |
| DOI | 10.1093/emboj/17.11.3207 |
| PMID | 9606202 |
| PMC | PMC1170659 |
| MeSH | Bacterial Proteins / biosynthesis Bacterial Proteins / genetics* Bacterial Proteins / metabolism DNA-Directed DNA Polymerase Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes, Bacterial* Mutagenesis Rec A Recombinases / antagonists & inhibitors* Rec A Recombinases / genetics Rec A Recombinases / metabolism Recombination, Genetic SOS Response, Genetics / genetics* |
| IF | 9.889 |
| 引用数 | 61 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9625 ME9626 ME9627 |