| RRC ID |
30159
|
| 著者 |
Yamazoe M, Onogi T, Sunako Y, Niki H, Yamanaka K, Ichimura T, Hiraga S.
|
| タイトル |
Complex formation of MukB, MukE and MukF proteins involved in chromosome partitioning in Escherichia coli.
|
| ジャーナル |
EMBO J
|
| Abstract |
mukF, mukE and mukB genes are essential for the process of chromosome partitioning in Escherichia coli. We have studied protein-protein interactions among MukB, MukE and MukF proteins by co-immunoprecipitation and sucrose gradient sedimentation experiments, using mukFEB null cells harboring plasmids carrying the wild-type or mutant-type mukFEB operon. MukB forms a complex with MukF and MukE. Analysis of mutant MukB proteins suggested that MukF and MukE bind the C-terminal globular domain of MukB. MukF is indispensable for an interaction between MukB and MukE; however, MukF itself is able to associate with MukB even in the absence of MukE. We have also found that MukF has a Ca(2+)-binding activity. Although purified MukF was able to make a complex either with MukE or MukB, a complex consisting of the three Muk proteins was barely detected in vitro. However, increasing the Ca(2+) or Mg(2+) concentration in the reaction partially restored complex formation. This suggests that Ca(2+) or Mg(2+) may be required for the formation of a complex consisting of the three Muk proteins, and thus may participate in a particular step during chromosome partitioning.
|
| 巻・号 |
18(21)
|
| ページ |
5873-84
|
| 公開日 |
1999-11-1
|
| DOI |
10.1093/emboj/18.21.5873
|
| PMID |
10545099
|
| PMC |
PMC1171653
|
| MeSH |
Amino Acid Sequence
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Calcium / metabolism
Centrifugation, Density Gradient
Chromosomal Proteins, Non-Histone*
Chromosomes / genetics*
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Escherichia coli / genetics
Escherichia coli / metabolism*
Escherichia coli Proteins*
Genes, Bacterial
Magnesium / metabolism
Molecular Sequence Data
Mutation
Precipitin Tests
Protein Binding
Repressor Proteins*
Sequence Alignment
|
| IF |
9.889
|
| 引用数 |
102
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME7831
ME7833
ME7836
ME7837
ME7838
ME7839
ME7821
ME7823
ME7834
ME7840
ME7822
ME7824
ME7830
ME7832
ME7799
ME7855
ME7856
ME7862
ME7854
ME7857
ME7858
ME7861
ME7863
ME7849
ME7850
ME7851
ME7852
ME7853 |
