論文 - 詳細
| RRC ID | 30159 |
|---|---|
| 著者 | Yamazoe M, Onogi T, Sunako Y, Niki H, Yamanaka K, Ichimura T, Hiraga S. |
| タイトル | Complex formation of MukB, MukE and MukF proteins involved in chromosome partitioning in Escherichia coli. |
| ジャーナル | EMBO J |
| Abstract |
mukF, mukE and mukB genes are essential for the process of chromosome partitioning in Escherichia coli. We have studied protein-protein interactions among MukB, MukE and MukF proteins by co-immunoprecipitation and sucrose gradient sedimentation experiments, using mukFEB null cells harboring plasmids carrying the wild-type or mutant-type mukFEB operon. MukB forms a complex with MukF and MukE. Analysis of mutant MukB proteins suggested that MukF and MukE bind the C-terminal globular domain of MukB. MukF is indispensable for an interaction between MukB and MukE; however, MukF itself is able to associate with MukB even in the absence of MukE. We have also found that MukF has a Ca(2+)-binding activity. Although purified MukF was able to make a complex either with MukE or MukB, a complex consisting of the three Muk proteins was barely detected in vitro. However, increasing the Ca(2+) or Mg(2+) concentration in the reaction partially restored complex formation. This suggests that Ca(2+) or Mg(2+) may be required for the formation of a complex consisting of the three Muk proteins, and thus may participate in a particular step during chromosome partitioning. |
| 巻・号 | 18(21) |
| ページ | 5873-84 |
| 公開日 | 1999-11-1 |
| DOI | 10.1093/emboj/18.21.5873 |
| PMID | 10545099 |
| PMC | PMC1171653 |
| MeSH | Amino Acid Sequence Bacterial Proteins / genetics Bacterial Proteins / metabolism* Calcium / metabolism Centrifugation, Density Gradient Chromosomal Proteins, Non-Histone* Chromosomes / genetics* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins* Genes, Bacterial Magnesium / metabolism Molecular Sequence Data Mutation Precipitin Tests Protein Binding Repressor Proteins* Sequence Alignment |
| IF | 9.889 |
| 引用数 | 102 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME7831 ME7833 ME7836 ME7837 ME7838 ME7839 ME7821 ME7823 ME7834 ME7840 ME7822 ME7824 ME7830 ME7832 ME7799 ME7855 ME7856 ME7862 ME7854 ME7857 ME7858 ME7861 ME7863 ME7849 ME7850 ME7851 ME7852 ME7853 |