論文 - 詳細
| RRC ID | 3017 |
|---|---|
| 著者 | Pikuta EV, Itoh T, Krader P, Tang J, Whitman WB, Hoover RB. |
| タイトル | Anaerovirgula multivorans gen. nov., sp. nov., a novel spore-forming, alkaliphilic anaerobe isolated from Owens Lake, California, USA. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel, alkaliphilic, obligately anaerobic bacterium, strain SCAT, was isolated from mud sediments of a soda lake in California, USA. The rod-shaped cells were motile, Gram-positive, formed spores and were 0.4-0.5x2.5-5.0 microm in size. Growth occurred within the pH range 6.7-10.0 and was optimal at pH 8.5. The temperature range for growth was 10-45 degrees C, with optimal growth at 35 degrees C. NaCl was required for growth. Growth occurred at 0.5-9.0% (w/v) NaCl and was optimal at 1-2% (w/v). The novel isolate was a catalase-negative chemo-organoheterotroph that fermented sugars, proteolysis products, some organic and amino acids, glycerol, d-cellobiose and cellulose. It was also capable of growth by the Stickland reaction. Strain SCAT was sensitive to tetracycline, chloramphenicol, rifampicin and gentamicin, but it was resistant to ampicillin and kanamycin. The G+C content of the genomic DNA was 34.2 mol%. Major fatty acid components were C14:0, iso-C15:0, C16:1omega9c and C16:0. 16S rRNA gene sequence analysis of strain SCAT showed a similarity of approximately 97% with the type strains of Clostridium formicaceticum and Clostridium aceticum in clostridial cluster XI and a similarity of less than 94.2% to any other recognized Clostridium species and those of related genera in this cluster. Strain SCAT was clearly differentiated from C. formicaceticum and C. aceticum based on comparison of their phenotypic properties and fatty acid profiles, as well as low levels of DNA-DNA relatedness between strain SCAT and the type strains of these two species. Therefore, strain SCAT is considered to represent a novel species of a new genus, Anaerovirgula multivorans gen. nov., sp. nov., in clostridial cluster XI. The type strain is SCAT (=ATCC BAA-1084T=JCM 12857T=DSM 17722T=CIP 107910T). |
| 巻・号 | 56(Pt 11) |
| ページ | 2623-2629 |
| 公開日 | 2006-11-1 |
| DOI | 10.1099/ijs.0.64198-0 |
| PMID | 17082402 |
| MeSH | Amino Acids / metabolism Anaerobiosis Anti-Bacterial Agents / pharmacology Bacterial Typing Techniques Base Composition California Carbohydrate Metabolism Carboxylic Acids / metabolism Catalase / analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Fatty Acids / chemistry Genes, rRNA Geologic Sediments / microbiology* Gram-Positive Endospore-Forming Rods / classification* Gram-Positive Endospore-Forming Rods / cytology Gram-Positive Endospore-Forming Rods / isolation & purification* Gram-Positive Endospore-Forming Rods / physiology Hydrogen-Ion Concentration Molecular Sequence Data Movement Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sodium Chloride / metabolism Temperature Water Microbiology |
| IF | 2.415 |
| 引用数 | 27 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | JCM 12857? |