Reference - Detail
| RRC ID | 30260 |
|---|---|
| Author | Yoshida Y, Kuroiwa H, Misumi O, Yoshida M, Ohnuma M, Fujiwara T, Yagisawa F, Hirooka S, Imoto Y, Matsushita K, Kawano S, Kuroiwa T. |
| Title | Chloroplasts divide by contraction of a bundle of nanofilaments consisting of polyglucan. |
| Journal | Science |
| Abstract |
In chloroplast division, the plastid-dividing (PD) ring is a main structure of the PD machinery and is a universal structure in the plant kingdom. However, the components and formation of the PD ring have been enigmatic. By proteomic analysis of PD machineries isolated from Cyanidioschyzon merolae, we identified the glycosyltransferase protein plastid-dividing ring 1 (PDR1), which constructs the PD ring and is widely conserved from red alga to land plants. Electron microscopy showed that the PDR1 protein forms a ring with carbohydrates at the chloroplast-division site. Fluorometric saccharide ingredient analysis of purified PD ring filaments showed that only glucose was included, and down-regulation of PDR1 impaired chloroplast division. Thus, the chloroplasts are divided by the PD ring, which is a bundle of PDR1-mediated polyglucan filaments. |
| Volume | 329(5994) |
| Pages | 949-53 |
| Published | 2010-8-20 |
| DOI | 10.1126/science.1190791 |
| PII | 329/5994/949 |
| PMID | 20724635 |
| MeSH | Algal Proteins / genetics Algal Proteins / isolation & purification Algal Proteins / physiology* Chloroplasts / chemistry Chloroplasts / physiology* Chloroplasts / ultrastructure Cytoskeleton / chemistry Cytoskeleton / physiology* Down-Regulation Glucans / isolation & purification Glucans / physiology* Glycosyltransferases / genetics Glycosyltransferases / isolation & purification Glycosyltransferases / physiology* Protein Binding Proteomics Rhodophyta / genetics Rhodophyta / physiology* Rhodophyta / ultrastructure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| IF | 41.846 |
| Times Cited | 55 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | F1000Research |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Algae | NIES-3377 |